Abstract
Helleborus genus, belonging to the Ranunculaceae family, has 20 species of herbaceous perennial flowering plants. The commercial exploitation of this plant is dependent on the selection and propagation of appropriate lines. High propagation rate could be accomplished by using a suitable tissue culture method enabling the rapid introduction of valuable selections in the market. However, in vitro cultivation of Helleborus is still very difficult. Thereby the development of reliable in vitro propagation procedures is crucial for future production systems. Axillary buds cultured on agar-solidified Murashige and Skoog medium supplemented with 1 mg/L benzyladenine, 0.1 mg/L β-naphthoxyacetic acid, and 2 mg/L isopentenyl adenine develop shoots after 16 weeks of culture under 16 h light regime, 50–60 μmol/s/m2, and 19 ± 1°C. The multiplication rate ranges from 1.4 to 2.1. However, the genotype and the number of subcultures affect the efficiency of the micropropagation process. The rooting of shoots is about 80% in solidified MS medium containing 1 mg/L 1-naphthaleneacetic acid and 3 mg/L indole-3-butyric acid. The described protocol provides information which can contribute to the commercial production of Helleborus plants.
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References
Springer P (1995) DE/GA Ausstellungen—IPM. Dtsch Gartenbau 8:477
Dan Y, Rothrock RE (2007) Development of an efficient micropropagation system for Helleborus. In Vitro Biology Meeting Abstract Issue p. 2022
Lim CC, Kitto SL (1995) Micropropagation of Helleborus orientalis Lam. and Aconitum uncinatum Linn. (Ranunculaceae). HortSci 30:871
Seyring M (2002) In vitro cloning of Helleborus niger. Plant Cell Rep 20:895–900
Poupet R, Cardin L, Henri A, Onesto JP (2006) Healthy in vitro propagation by meristem tip culture of Helleborus niger’s selected clone for cut flower. Acta Hortic 725:301–310
Dhooghe E, Van Labeke MC (2007) In vitro propagation of Helleborus species. Plant Cell Tissue Organ Cult 91(2):175–177
Beruto M, Curir P (2009) Effects of chilling and hormonal supply on rooting and In vivo establishment of micropropagated plantlets of Helleborus spp. Acta Hortic 812:365–372
Colston Burrell C, Knott Tyler J (2006) Hellebores: a comprehensive guide. Timber press, Portland
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Quoirin M, Lepoivre P (1977) Étude de milieux adaptés aux cultures in vitro de Prunus. Acta Hortic 78:437–442
Jay-Allemand C, Capelli P, Cornu D (1992) Root development of in vitro hybrid walnut microcuttings in a vermiculite-containing gelrite medium. Sci Hortic 15:335–342
Lloyd G, Mc Cown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalima latifolia, by use of shoot tip culture. Pro Intl Plant Prop Soc 30:421–427
Acknowledgements
This work has been partially supported by the Project: INNORNA (D.M.19146/7643/08) (2009–2012), The Italian Ministry of Agriculture (MIPAF).
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Beruto, M., Viglione, S., Bisignano, A. (2012). Micropropagation of Helleborus through Axillary Budding. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_20
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DOI: https://doi.org/10.1007/978-1-62703-074-8_20
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Publisher Name: Humana Press, Totowa, NJ
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