Abstract
Automated multicolor fluorescence microscopy facilitates high-throughput quantitation of cellular parameters of complex, organotypic systems. In vitro co-cultured vascular cells form capillary-like networks that model facets of angiogenesis, making it an attractive alternative for anti-angiogenic drug discovery. We have adapted this angiogenesis assay system to a high-throughput format to enable automated image-based high-throughput screening of live primary human vascular cell co-cultures with chemical libraries for anti-angiogenic drug discovery. Protocols are described for setup of a fluorescence-based co-culture assay, live cell image acquisition, image analysis of morphological parameters, and screening data handling.
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Evensen, L., Link, W., Lorens, J.B. (2012). Image-Based High-Throughput Screening for Inhibitors of Angiogenesis. In: Taatjes, D., Roth, J. (eds) Cell Imaging Techniques. Methods in Molecular Biology, vol 931. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-056-4_8
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DOI: https://doi.org/10.1007/978-1-62703-056-4_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-055-7
Online ISBN: 978-1-62703-056-4
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