Abstract
Plant organ phenotyping by noninvasive video imaging techniques provides a powerful tool to assess physiological traits, circadian and diurnal rhythms, and biomass production. In particular, growth of individual plant organs is known to exhibit a high plasticity and occurs as a result of the interaction between various endogenous and environmental processes. Thus, any investigation aiming to unravel mechanisms that determine plant or organ growth has to accurately control and document the environmental growth conditions. Here we describe challenges in establishing a recently developed plant root monitoring platform (PlaRoM) specially suited for noninvasive high-throughput plant growth analysis with highest emphasis on the detailed documentation of capture time, as well as light and temperature conditions. Furthermore, we discuss the experimental procedure for measuring root elongation kinetics and key points that must be considered in such measurements. PlaRoM consists of a robotized imaging platform enclosed in a custom designed phytochamber and a root extension profiling software application. This platform has been developed for multi-parallel recordings of root growth phenotypes of up to 50 individual seedlings over several days, with high spatial and temporal resolution. Two Petri dishes are mounted on a vertical sample stage in a custom designed phytochamber that provides exact temperature control. A computer-controlled positioning unit moves these Petri dishes in small increments and enables continuous screening of the surface under a binocular microscope. Detection of the root tip is achieved by applying thresholds on image pixel data and verifying the neighbourhood for each dark pixel. The growth parameters are visualized as position over time or growth rate over time graphs and averaged over consecutive days, light–dark periods and 24 h day periods. This setup enables the investigation of root extension profiles of different genotypes in various growth conditions (e.g., light protocol, temperature, growth media) and is especially suited for the detection of diurnal or circadian growth rhythms.
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References
Yazdanbakhsh N, Fisahn J (2010) Analysis of Arabidopsis thaliana root growth kinetics with high temporal and spatial resolution. Ann Bot 105:783–791
Yazdanbakhsh N, Fisahn J (2009) High-throughput phenotyping of root growth dynamics, lateral root formation, root architecture and root hair development enabled by PlaRoM. Funct Plant Biol 36:938–946
Yazdanbakhsh N, Sulpice R, Graf A, Stitt M, Fisahn J (2011) Circadian control of root elongation and C partitioning in Arabidopsis thaliana. Plant Cell Environ 34:877–894
Yazdanbakhsh N, Fisahn J (2011) Stable diurnal growth rhythms modulate root elongation of Arabidopsis thaliana. Plant Root 5:17–23
Yazdanbakhsh N, Fisahn J (2011) Mutations in leaf starch metabolism modulate the diurnal root growth profiles of Arabidopsis thaliana. Plant Signal Behav 6:1–4
Iijima M, Matsushita N (2011) A circadian and an ultradian rhythm are both evident in root growth of rice. J Plant Physiol. doi:10.1016/j.jplph.2011.06.005
Yazdanbakhsh N, Fisahn J (2007) Investigation of plant root elongation by screening the surface of a Petri dish. In: Arabnia HR (ed) Proceedings of the 2007 international conference on image processing, computer vision and pattern recognition. CSREA Press
Yazdanbakhsh N, Fisahn J (2007) Development of a robot based platform applied to simultaneous root growth profiling of seedlings growing in a Petri dish. In: Aggarwal A, Yager R, Sandberg IW (eds) Proceedings of the 8th WSEAS international conference on mathematics and computers in biology and chemistry (MCBC’07). World Scientific and Engineering Academy and Society Press
Acknowledgments
Development of the described platform was supported by the Max Planck Society and by a contract to N.Y. We appreciate the permissions of Oxford University Press and CSIRO Publishing to reproduce some parts of our figures that were originally published in Annals of Botany and Functional Plant Biology.
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Yazdanbakhsh, N., Fisahn, J. (2012). High-Throughput Phenotyping of Root Growth Dynamics. In: Normanly, J. (eds) High-Throughput Phenotyping in Plants. Methods in Molecular Biology, vol 918. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-995-2_3
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DOI: https://doi.org/10.1007/978-1-61779-995-2_3
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Publisher Name: Humana Press, Totowa, NJ
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