Abstract
Antibody–antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K D can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature-dependent antibody kinetics is recognized, especially as part of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method.
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Schräml, M., von Proff, L. (2012). Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection. In: Proetzel, G., Ebersbach, H. (eds) Antibody Methods and Protocols. Methods in Molecular Biology, vol 901. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-931-0_12
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DOI: https://doi.org/10.1007/978-1-61779-931-0_12
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-930-3
Online ISBN: 978-1-61779-931-0
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