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Methodological Approaches to Cytochrome P450 Profiling in Embryos

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Developmental Toxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 889))

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Abstract

Cytochrome P450 enzymes (CYPs) are heme thiolate proteins essential for vertebrate development and also play important roles in toxicology as well as normal metabolic function. CYP enzymes catalyze the oxidative biotransformation of many endogenous and exogenous chemicals, including steroids and eicosanoids, and drugs and other xenobiotic toxicants. Many CYPs have known for their potential roles in development, and many chemicals that cause developmental abnormalities are substrates for CYPs. The roles and regulation of most xenobiotic metabolizing CYPs during development are unknown, impeding understanding of mechanisms of developmental toxicity. The zebrafish (Danio rerio) has become one of the premier models in vertebrate developmental biology, in large part because of the logistical advantages of rapid external development, high adult maintenance density, and significant (bio)technological tool availability. The increasing use of zebrafish in drug discovery and mechanistic toxicology demands knowledge of CYP gene regulation and function. Here, we present methods to examine CYP expression during early development in zebrafish, with an emphasis on developmental microarrays.

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Acknowledgments

This work was supported by NIH grants R01ES015912 and P42ES007381 (Superfund Basic Research Program at Boston University).

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Authors and Affiliations

  1. Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA, USA

    Jared V. Goldstone Ph.D.

  2. Department of Biology, Woods Hole Oceanographic Institute, Woods Hole, MA, USA

    John J. Stegeman Ph.D.

Authors
  1. Jared V. Goldstone Ph.D.
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  2. John J. Stegeman Ph.D.
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Corresponding author

Correspondence to Jared V. Goldstone Ph.D. .

Editor information

Editors and Affiliations

  1. Toxicology Program, Dept. Environmental Health Sciences, University of Michigan, Washington Heights 1420, Ann Arbor, 48109-2029, Michigan, USA

    Craig Harris

  2. , Department of Pediatrics, Emory University, Uppergate Drive NE., 2015Suite 350, Atlanta, 30322, Georgia, USA

    Jason M. Hansen

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© 2012 Springer Science+Business Media, LLC

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Goldstone, J.V., Stegeman, J.J. (2012). Methodological Approaches to Cytochrome P450 Profiling in Embryos. In: Harris, C., Hansen, J. (eds) Developmental Toxicology. Methods in Molecular Biology, vol 889. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-867-2_16

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  • DOI: https://doi.org/10.1007/978-1-61779-867-2_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-866-5

  • Online ISBN: 978-1-61779-867-2

  • eBook Packages: Springer Protocols

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