Abstract
Rhodopsin is the dim-light photoreceptor responsible for initiation of the visual transduction cascade. In the dark its activity is very low, while light activation catalyzes the activation of its G-protein transducin. The first step in resetting rhodopsin and the phototransduction cascade involves the phosphorylation of light-active rhodopsin by rhodopsin kinase. Here, we describe assays to monitor the function of rhodopsin or rhodopsin mutants.
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Sammons, J., Gross, A.K. (2012). In Vitro Biochemical Assays to Monitor Rhodopsin Function. In: Wang, SZ. (eds) Retinal Development. Methods in Molecular Biology, vol 884. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-848-1_11
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DOI: https://doi.org/10.1007/978-1-61779-848-1_11
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Publisher Name: Humana Press, Totowa, NJ
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