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HLA Typing by SSO and SSP Methods

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Immunogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 882))

Abstract

Human leukocyte antigen (HLA) typing, utilising the sequence-specific oligonucleotide (SSO) and sequence-specific primer (SSP) technologies, has been in routine use in many tissue typing laboratories worldwide for more than 20 years since the development of the polymerase chain reaction. Both methods are very useful for clinical and research purposes and can provide generic (low resolution) to allelic (high resolution) typing results. This chapter provides an overview of the SSO and SSP methods in relation to HLA typing.

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References

  1. Olerup O, Zetterquist H (1992) HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 39:225–235

    Article  PubMed  CAS  Google Scholar 

  2. Olerup O, Aldener A, Fogdell A (1993) HLA-DQB1 and -DQA1 typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours. Tissue Antigens 41:119–134

    Article  PubMed  CAS  Google Scholar 

  3. Bunce M, Taylor CJ, Welsh KI (1993) Rapid HLA-DQB typing by eight polymerase chain reaction amplifications with sequence-specific primers (PCR-SSP). Hum Immunol 37:201–206

    Article  PubMed  CAS  Google Scholar 

  4. Browning MJ, Krausa P, Rowan A et al (1993) Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. Proc Natl Acad Sci USA 90:2842–2845

    Article  PubMed  CAS  Google Scholar 

  5. Sadler AM, Petronzelli F, Krausa P et al (1994) Low-resolution DNA typing for HLA-B using sequence-specific primers in allele- or group-specific ARMS/PCR. Tissue Antigens 44:148–154

    Article  PubMed  CAS  Google Scholar 

  6. Bunce M, O’Neill CM, Barnardo MCNM et al (1995) Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilising sequence-specific primers (PCR-SSP). Tissue Antigens 46:355–367

    Article  PubMed  CAS  Google Scholar 

  7. Wordsworth BP, Allsopp CE, Young RP et al (1990) HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes. Immunogenetics 32:413–418

    Article  PubMed  CAS  Google Scholar 

  8. Morel C, Zwahlen F, Jeannet M et al (1990) Complete analysis of HLA-DQB1 polymorphism and DR-DQ linkage disequilibrium by oligonucleotide typing. Hum Immunol 29:64–77

    Article  PubMed  CAS  Google Scholar 

  9. Scharf SJ, Griffith RL, Erlich HA (1991) Rapid typing of DNA sequence polymorphism at the HLA-DRB1 locus using the polymerase chain reaction and nonradioactive oligonucleotide probes. Hum Immunol 30:190–201

    Article  PubMed  CAS  Google Scholar 

  10. Kimura A, Sasazuki T (1992) Eleventh international histocompatibility workshop reference protocol for the HLA-DNA typing technique. In: Tsuji K, Aizawa M, Sasazuki T (eds) HLA 1991. Oxford University Press, Oxford, p 397

    Google Scholar 

  11. Gao X, Jakobsen IB, Serjeantson SW (1994) Characterization of the HLA-A polymorphism by locus-specific polymerase chain reaction amplification and oligonucleotide hybridization. Hum Immunol 41:267–279

    Article  PubMed  CAS  Google Scholar 

  12. Cereb N, Yang SY (1997) Dimorphic primers derived from intron 1 for use in the molecular typing of HLA-B alleles. Tissue Antigens 50:74–76

    Article  PubMed  CAS  Google Scholar 

  13. Erlich H, Bugawan T, Begovich AB et al (1991) HLA-DR, DQ and DP typing using PCR amplification and immobilized probes. Eur J Immunogenet 18:33–55

    Article  PubMed  CAS  Google Scholar 

  14. Bugawan TL, Apple R, Erlich HA (1994) A method for typing polymorphism at the HLA-A locus using PCR amplification and immobilized oligonucleotide probes. Tissue Antigens 44:137–147

    Article  PubMed  CAS  Google Scholar 

  15. Robinson J, Malik A, Parham P et al (2000) IMGT/HLA—a sequence database for the human major histocompatibility complex. Tissue Antigens 55:280–287. http://www.ebi.ac.uk/imgt/hla/

    Google Scholar 

  16. Robinson J, Mistry K, McWilliam H et al (2011) The IMGT/HLA database. Nucleic Acids Res 39(suppl 1):D1171–D1176

    Article  PubMed  Google Scholar 

  17. Mytilineos J, Scherer S, Dunckley H et al (1993) DNA HLA-DR typing results of 4000 kidney transplants. Transplantation 55:778–781

    Article  PubMed  CAS  Google Scholar 

  18. Mytilineos J, Lempert M, Middleton D et al (1997) HLA class I DNA typing of 215 “HLA-A, -B, -DR zero mismatched” kidney transplants. Tissue Antigens 50:355–358

    Article  PubMed  CAS  Google Scholar 

  19. Lorentzen DF, Iwanaga KK, Meuer KJ et al (1997) 25% Error rate in serologic typing of HLA-B homozygotes. Tissue Antigens 50:359–365

    Article  PubMed  CAS  Google Scholar 

  20. Mytilineos J, Christ U, Lempert M et al (1997) Comparison of typing results by serology and polymerase chain reaction with sequence-specific primers for HLA-Cw in 650 individuals. Tissue Antigens 50:395–400

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Australian Red Cross Blood Service, Sydney, NSW, Australia

    Heather Dunckley

Authors
  1. Heather Dunckley
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Corresponding author

Correspondence to Heather Dunckley .

Editor information

Editors and Affiliations

  1. PathWest Laboratory Medicine, Department of Clinical Immunology, Royal Perth Hospital, Wellington Street, Perth, 6000, Australia

    Frank T. Christiansen

  2. Australian Red Cross Blood Service, National Transplant Services, The Royal Melbourne Hospital, 150-154 Batman Street, Melbourne VIC, 3000, Australia

    Brian D. Tait

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Dunckley, H. (2012). HLA Typing by SSO and SSP Methods. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_2

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  • DOI: https://doi.org/10.1007/978-1-61779-842-9_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-841-2

  • Online ISBN: 978-1-61779-842-9

  • eBook Packages: Springer Protocols

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