Abstract
This chapter discusses, briefly, the developments in electrophoresis of proteins from Tiselius’ moving boundary electrophoresis to the modern day two-dimensional polyacrylamide gel electrophoresis. It also touches upon the staining methods used to visualize total proteins postelectrophoresis.
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References
Tiselius A (1938) Elektrophoretische Messungen am Eiweiss. Kolloid Z 85:129
Righetti PG (2005) Electrophoresis: the march of pennies, the march of dimes. J Chromatogr A 1079:24–40
Von Klobusitzky P, Konig P (1939) Biochemische studien uber die gifte der schlan gengattung. Arch Exp Path Pharmakol 192:271–275
Evered DF (1959) Ionophoresis of acidic and basic amino acids on filter paper using low voltages. Biochim Biophys Acta 36:14–19
Ingram VM (1958) The comparison of normal human and sicke-cell haemoglobins by fingerprinting. Biochim Biophys Acta 28:539–545
Smithies O (1955) Zone electrophoresis in starch gels: group variations in serum proteins of normal human adults. Biochem J 61:629–641
Poulik MD (1957) Starch gel electrophoresis in a discontinuous system of buffers. Nature 180:1477–1479
Raymond S, Weintraub L (1959) Acrylamide gel as a supporting medium for zone electrophoresis. Science 1959(130):711–712
Raymond S (1964) Acrylamide gel electrophoresis. Ann N Y Acad Sci 121:35–65
Ornstein L (1964) Disc electrophoresis. I. Background and theory. Ann N Y Acad Sci 28:32–49
Gelfi C, Righetti PG (1981) Polymerization kinetics of polyacrylamide gels I. Effect of different cross-linkers. Electrophoresis 2:213–219
Righetti PG, Gelfi C, Bosisio AB (1981) Polymerization kinetics of polyacrylamide gels III. Effect of catalysis. Electrophoresis 2: 291–295
Andrews AT (1986) Polyacrylamide gel electrophoresis (PAGE): homogeneous gel and buffer systems. In: Electrophoresis: theory, techniques, and biochemical and clinical applications. Oxford University Press, New York
Maurer HR (1971) Disc electrophoresis and related techniques of polyacrylamide gel electrophoresis, 2nd edn. Walter de Gruyter, Berlin
Margolis J, Kenrick KG (1967) Polyacrylamide gel-electrophoresis across a molecular sieve gradient. Nature 214:1334–1336
Margolis J, Kenrick KG (1967) Electrophoresis in polyacrylamide concentration gradient. Biochem Biophys Res Commun 27:68–73
Shapiro AL, Vinuela E, Maizel JV (1967) Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun 28: 815–820
Ornstein L (1964) Disc electrophoresis. I. Background and theory. Ann N Y Acad Sci 121:321–349
Davis BJ (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci 121:404–427
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Steinberg TH (2009) Protein gel staining methods: an introduction and overview. Methods Enzymol 463:541–563
Neuhoff V, Stamm R, Hansjorg E (1985) Clear background and highly sensitive protein staining with coomassie blue dyes in polyacrylamide gels: a systematic analysis. Electrophoresis 6:427–488
Switzer RC, Merril CR, Shifrin S (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem 98:231–237
Daban J-R, Bartolome S, Samso M (1991) Use of the hydrophobic probe Nile red for the fluorescent staining of protein bands in sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem 199:169–174
Berggren K, Chernokalskaya E, Steinberg TH et al (2000) Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex. Electrophoresis 21:2509–2521
Mackintosh JA, Choi H-Y, Bae S-H et al (2003) A fluorescent natural product for ultrasensitive detection of proteins in one-dimensional and two-dimensional gel electrophoresis. ProteomÂics 3:2273–2288
Steinberg TH, Agnew BJ, Gee KR et al (2003) Global quantitative phosphoprotein analysis using multiplexed proteomics technology. Proteomics 3:1128–1144
Kinoshita E, Kinoshita-Kikuta E, Takiyama K, Koike T (2006) Phosphate binding tag, a new tool to visualize phosphorylated proteins. Mol Cell Proteomics 5:749–757
O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
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Srinivas, P.R. (2012). Introduction to Protein Electrophoresis. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_2
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DOI: https://doi.org/10.1007/978-1-61779-821-4_2
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