Abstract
Although stem cells are commonly isolated by fluorescence-activated cell sorting or magnetic affinity cell sorting, they are very expensive, and they need known markers. However, there is no specific marker for liver stem/progenitor cells (LSPCs). Here, we describe a convenient and efficient method (three-step method) to enrich LSPCs. The fetal liver cells (FLCs) were firstly enriched by Percoll discontinuous gradient centrifugation from the rat fetal liver. Then the FLCs in culture were purified to be homogeneous in size by differential trypsinization and differential adherence. Finally, fetal liver stem/progenitor cells (FLSPCs) were enriched from purified FLCs by Percoll continuous gradient centrifugation. Flow cytometric analysis combining with marker CD133 was used to detect the purity of FLSPCs and evaluate the isolating effects of the three-step method.
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Acknowledgments
Our study was supported by the National Natural Science Foundation of China (NO30772102). We thank Fu-qin Zhang (the Fourth Military Medical University, China), who gave us a lot of help in the experimental design. Sincere thanks also go to Rui Guo (Sichuan International Studies of University, China) for language organizing.
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Liu, W., You, N., Dou, K. (2012). Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_9
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DOI: https://doi.org/10.1007/978-1-61779-815-3_9
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Publisher Name: Humana Press, Totowa, NJ
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