Abstract
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Here, we describe a method for efficient isolation and expansion of human cardiomyocyte progenitor cells (CMPCs) from cardiac surgical waste or, alternatively, from fetal heart tissue. Additionally, we provide a detailed in vitro protocol for efficient differentiation of CMPCs into cardiomyocytes with great efficiency (80–90% of differentiation). Once CMPCs are rapidly dividing (approximately 1 month after isolation), differentiation can be achieved in 3–4 weeks.
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Smits, A.M., van Oorschot, A.A., Goumans, MJ. (2012). Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells into Cardiomyocytes. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_20
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DOI: https://doi.org/10.1007/978-1-61779-815-3_20
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-814-6
Online ISBN: 978-1-61779-815-3
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