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Photosynthetic Antenna Complex LHCII Studied with Novel Fluorescence Techniques

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Spectroscopic Methods of Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 875))

Abstract

LHCII is the largest light-harvesting pigment-protein complex of plants, comprising more than half of photosynthetically active chlorophyll pigments in biosphere. Understanding relationship between the molecular structure of the complex and photophysical processes that undergo in this pigment-protein complex is an aim of numerous current studies. This chapter addresses possibility of the application of single-molecule fluorescence measurements and fluorescence lifetime imaging microscopy (FLIM) in a study of LHCII.

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References

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Acknowledgments

The research on LHCII is financed by the Ministry of Science and Higher Education of Poland from the funds for science in the years 2008–2011 within the research project N N303 285034. RL acknowledges the postdoctoral fellowship from the Ministry of Science and Higher Education of Poland (grant No.17/MOB/2007/0).

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Authors and Affiliations

  1. Department of Biophysics, Institute of Physics, Maria Curie-Sklodowska University, Lublin, Poland

    Wieslaw I. Gruszecki, Rafal Luchowski & Wojciech Grudzinski

  2. Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, USA

    Zygmunt Gryczynski & Ignacy Gryczynski

Authors
  1. Wieslaw I. Gruszecki
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  2. Rafal Luchowski
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  3. Wojciech Grudzinski
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  4. Zygmunt Gryczynski
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  5. Ignacy Gryczynski
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Corresponding author

Correspondence to Wieslaw I. Gruszecki .

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Editors and Affiliations

  1. Dept. Biochemistry & Molecular Biology, University of Texas Medical Branch, University Blvd. 301, Galveston, 77555-1053, Texas, USA

    Wlodek M. Bujalowski

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Gruszecki, W.I., Luchowski, R., Grudzinski, W., Gryczynski, Z., Gryczynski, I. (2012). Photosynthetic Antenna Complex LHCII Studied with Novel Fluorescence Techniques. In: Bujalowski, W. (eds) Spectroscopic Methods of Analysis. Methods in Molecular Biology, vol 875. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-806-1_14

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  • DOI: https://doi.org/10.1007/978-1-61779-806-1_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-805-4

  • Online ISBN: 978-1-61779-806-1

  • eBook Packages: Springer Protocols

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