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Phospholipase A2 Purification and Characterization: A Case Study

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Lipases and Phospholipases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 861))

Abstract

We compared here the purification procedures, the pH, the calcium, the bile salts, and the temperature dependencies as well as the catalytic activities on phosphatidylcholine (PC) and phosphatidylethanolamine (PE) of two purified secreted PLA2 from chicken pancreatic (ChPLA2-IB) and chicken intestinal (ChPLA2-IIA) origins. Interestingly, ChPLA2-IB hydrolyzes efficiently both purified PC and PE, whereas ChPLA2-IIA hydrolyzes only PE and not PC, even after a long incubation period. These analytical results clearly indicate that the catalytic activity of ChPLA2-IIA, measured with the pH-stat and using egg yolk as substrate, is mainly due to the hydrolysis of the PE fraction present in egg yolk.

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Author information

Authors and Affiliations

  1. Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d’Ingénieurs de Sfax, Sfax, Tunisia

    Aida Karray, Youssef Gargouri & Sofiane Bezzine

  2. Enzymology at interfaces and physiology of lipolysis, CNRS-Aix-Marseille Université, UPR 9025, Marseille Cedex 20, France

    Robert Verger

  3. National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia

    Sofiane Bezzine

Authors
  1. Aida Karray
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  2. Youssef Gargouri
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  3. Robert Verger
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  4. Sofiane Bezzine
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Corresponding author

Correspondence to Robert Verger .

Editor information

Editors and Affiliations

  1. CIATEJ-CONACYT, Industrial Biotechnology Unit, Avda. Normalistas 800, Guadalajara, 44270, Jalisco, Mexico

    Georgina Sandoval

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Karray, A., Gargouri, Y., Verger, R., Bezzine, S. (2012). Phospholipase A2 Purification and Characterization: A Case Study. In: Sandoval, G. (eds) Lipases and Phospholipases. Methods in Molecular Biology, vol 861. Humana Press. https://doi.org/10.1007/978-1-61779-600-5_17

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  • DOI: https://doi.org/10.1007/978-1-61779-600-5_17

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-599-2

  • Online ISBN: 978-1-61779-600-5

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