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DNA Barcoding in Mammals

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DNA Barcodes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 858))

Abstract

DNA barcoding provides an operational framework for mammalian taxonomic identification and cryptic species discovery. Focused effort to build a reference library of genetic data has resulted in the assembly of over 35 K mammalian cytochrome c oxidase subunit I sequences and outlined the scope of mammal-related barcoding projects. Based on the above experience, this chapter recounts three typical methodological pathways involved in mammalian barcoding: routine methods aimed at assembling the reference sequence library from high quality samples, express approaches used to attain cheap and fast taxonomic identifications for applied purposes, and forensic techniques employed when dealing with degraded material. Most of the methods described are applicable to a range of vertebrate taxa outside Mammalia.

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Acknowledgments

We thank Judith Eger, Mark Engstrom, Burton Lim, Don Stewart, Charles Francis, Sergey Kruskop, Andrey Lissovsky, Vladimir Lebedev, Natalia Abramson, Ivan Kuzmin, Bernard Agwanda, Anna Bannikova, Ticul Alvarez, Fernando Cervantez, Curtis Strobeck, Jack Millar, and William Pruitt, Jr. for providing materials for analysis; Robert Hanner for advice on protocol development; Agata Pawlowski and Miranda Elliott for protocol testing; and Paul Hebert for administrative support. DNA analyses were performed at the Canadian Centre of DNA Barcoding, Biodiversity Institute of Ontario, and University of Guelph, and supported by grants to Paul Hebert from the Gordon and Betty Moore Foundation, Genome Canada through the Ontario Genomics Institute, (2008-OGI-ICI-03) the Canada Foundation for Innovation, the Ontario Innovation Trust, and the Natural Sciences and Engineering Research Council of Canada.

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Authors and Affiliations

  1. Biodiversity Institute of Ontario & Integrative Biology, University of Guelph, Guelph, ON, Canada

    Natalia V. Ivanova, Elizabeth L. Clare & Alex V. Borisenko

Authors
  1. Natalia V. Ivanova
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  2. Elizabeth L. Clare
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  3. Alex V. Borisenko
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Corresponding author

Correspondence to Alex V. Borisenko .

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Editors and Affiliations

  1. National Museum of Natural History, Dept. Botany, Smithsonian Institution, 10th St. & Constitution Ave. NW.,, Washington, 20560-0166, District of Columbia, USA

    W. John Kress

  2. National Museum of Natural History, Dept. Botany, Smithsonian Institution, 10th St. & Constitution Ave. NW.,, Washington, 20560-0166, District of Columbia, USA

    David L. Erickson

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Ivanova, N.V., Clare, E.L., Borisenko, A.V. (2012). DNA Barcoding in Mammals. In: Kress, W., Erickson, D. (eds) DNA Barcodes. Methods in Molecular Biology, vol 858. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-591-6_8

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  • DOI: https://doi.org/10.1007/978-1-61779-591-6_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-590-9

  • Online ISBN: 978-1-61779-591-6

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