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DNA Barcoding Fishes

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DNA Barcodes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 858))

Abstract

This chapter is an overview of the techniques for DNA barcoding of fishes from field collection to DNA sequence analysis. Recommendations for modifications of field protocols and best tissue sampling practices are made. A variety of DNA extraction protocols is provided, including high-throughput robot-assisted methods. A pair of well-tested forward and reverse primers for PCR amplification and sequencing are presented. These primers have been successfully used for DNA barcode on a wide array of marine fish taxa and also work well in most freshwater and cartilaginous fishes. Recipes and cycling protocols for both PCR amplification and sequencing and cleanup methods for the reaction products are provided. A method for the consistent production of high-quality DNA barcodes from DNA sequence data is given and stringent guidelines for judging the quality of raw sequence data are laid out.

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Acknowledgments

Our Fish DNA Barcoding Team would like to thank the NMNH Fish scientists and taxonomists for taking the time to educate us on fish biology and biodiversity and make this work thoroughly enjoying. We also thank David Erickson for his tireless efforts to produce this important volume.

Author information

Authors and Affiliations

  1. Laboratories of Analytical Biology, Smithsonian Institution, NMNH, Suitland, MD, USA

    Lee A. Weigt, Amy C. Driskell & Andrea Ormos

  2. Department of Vertebrate Zoology, National Museum of Natural History, Suitland, MD, USA

    Carole C. Baldwin

Authors
  1. Lee A. Weigt
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  2. Amy C. Driskell
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  3. Carole C. Baldwin
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  4. Andrea Ormos
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Corresponding author

Correspondence to Lee A. Weigt .

Editor information

Editors and Affiliations

  1. National Museum of Natural History, Dept. Botany, Smithsonian Institution, 10th St. & Constitution Ave. NW.,, Washington, 20560-0166, District of Columbia, USA

    W. John Kress

  2. National Museum of Natural History, Dept. Botany, Smithsonian Institution, 10th St. & Constitution Ave. NW.,, Washington, 20560-0166, District of Columbia, USA

    David L. Erickson

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© 2012 Springer Science+Business Media, LLC

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Weigt, L.A., Driskell, A.C., Baldwin, C.C., Ormos, A. (2012). DNA Barcoding Fishes. In: Kress, W., Erickson, D. (eds) DNA Barcodes. Methods in Molecular Biology, vol 858. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-591-6_6

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  • DOI: https://doi.org/10.1007/978-1-61779-591-6_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-590-9

  • Online ISBN: 978-1-61779-591-6

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