Abstract
Molecular barcoding is an essential tool to use the high throughput of next generation sequencing platforms optimally in studies involving more than one sample. Various barcoding strategies allow for the incorporation of short recognition sequences (barcodes) into sequencing libraries, either by ligation or polymerase chain reaction (PCR). Here, we present two approaches optimized for generating barcoded sequencing libraries from low copy number extracts and amplification products typical of ancient DNA studies.
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Acknowledgments
This work was supported by the Max Planck Society, the German Research Foundation (DFG), the Allan Wilson Centre for Molecular Ecology and Evolution, the University of Otago and Pennsylvania State University.
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Knapp, M., Stiller, M., Meyer, M. (2012). Generating Barcoded Libraries for Multiplex High-Throughput Sequencing. In: Shapiro, B., Hofreiter, M. (eds) Ancient DNA. Methods in Molecular Biology, vol 840. Humana Press. https://doi.org/10.1007/978-1-61779-516-9_19
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DOI: https://doi.org/10.1007/978-1-61779-516-9_19
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