Abstract
Primary keratinocytes are an important tool to investigate the molecular mechanism underlying the skin phenotype of mice with null mutations in Rho GTPase genes. If the RhoA gene deletion is conditional, the knockout can be induced in vitro by transfection with cre-IRES-GFP and sorting for GFP positive cells by flow cytometry. Such in vitro knockout will allow determining the cell autonomous functions of the Rho GTPase, independent of any in vivo interactions. Using the same method, also other expression vectors or knockdown constructs can be introduced into primary mouse keratinocytes.
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Acknowledgments
This work was supported by the Novo Nordisk Foundation, the Lundbeck Foundation, and the Danish Research Council.
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Pedersen, E., Basse, A., Lefever, T., Peyrollier, K., Brakebusch, C. (2012). Rho GTPase Knockout Induction in Primary Keratinocytes from Adult Mice. In: Rivero, F. (eds) Rho GTPases. Methods in Molecular Biology, vol 827. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-442-1_11
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DOI: https://doi.org/10.1007/978-1-61779-442-1_11
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