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Analysis of Transcriptional Regulation in Bone Cells

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

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Abstract

Transcription is the process by which the rate of RNA synthesis is regulated. Here, we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation assays, three commonly used methods for studying gene transcription.

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Author information

Authors and Affiliations

  1. Kennedy Institute of Rheumatology, London, UK

    Huilin Jin

  2. Rheumatic Diseases Unit, Western General Hospital, University of Edinburgh, Edinburgh, UK

    Stuart H. Ralston

Authors
  1. Huilin Jin
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  2. Stuart H. Ralston
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Corresponding author

Correspondence to Huilin Jin .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, University of Aberdeen, Forester Hill, Aberdeen, AB25 2ZD, United Kingdom

    Miep H. Helfrich

  2. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, United Kingdom

    Stuart H. Ralston

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© 2012 Springer Science+Business Media, LLC

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Jin, H., Ralston, S.H. (2012). Analysis of Transcriptional Regulation in Bone Cells. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_16

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

  • eBook Packages: Springer Protocols

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