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Light-Regulated Gene Expression in Yeast

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Synthetic Gene Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 813))

Abstract

An important basic requirement of synthetic genetic networks is the option of external control of gene expression. Although several chemically inducible systems are available, all of these suffer from the common problem: the chemical inducers are difficult to remove so that to terminate the response. We have described a regulatory expression system for yeast, which employs light as inducer. This light switch translates light-controlled protein–protein interactions into the transcription of selected genes in a dose-dependent and reversible manner.

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Correspondence to Ferenc Nagy .

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Kozma-Bognar, L., Hajdu, A., Nagy, F. (2012). Light-Regulated Gene Expression in Yeast. In: Weber, W., Fussenegger, M. (eds) Synthetic Gene Networks. Methods in Molecular Biology, vol 813. Humana Press. https://doi.org/10.1007/978-1-61779-412-4_11

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  • DOI: https://doi.org/10.1007/978-1-61779-412-4_11

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-411-7

  • Online ISBN: 978-1-61779-412-4

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