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Glycoarray by DNA-Directed Immobilization

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Carbohydrate Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 808))

Abstract

Glycoarrays have become a powerful platform to investigate the interactions of many biological events involving carbohydrates. The carbohydrates immobilization on the surface of the substrates is a key step of glycoarray fabrication. Plenty of strategies have been applied to the immobilization process. Herein a protocol for the synthesis of oligonucleotide glycomimetic conjugates is proposed. The resulting molecules are immobilized by hybridization on a DNA microarray (DNA-directed immobilization; DDI). DDI has been proved to be a very efficient and site-selective. This protocol provides detailed procedures for the preparation of fluorescent oligonucleotide trigalactosylmimetic conjugates and for the preparation of carbohydrate microarrays by DDI on glass slides.

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Acknowledgments

The authors thank the financial support from the CNRS, Ecole Centrale de Lyon, Université Claude Bernard Lyon 1, and Université Montpellier 2, “Interface Physique Chimie Biologie: soutien à la prise de risque,” ANR-08-BLAN-0114-01 and Lyon Biopole, Région Rhône–Alpes programme Cible 2010 and the Chinese Scientific Council for the award of a research studentship and Scholarship. The NanoLyon Platefrom is acknowledged for technical support.

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Authors and Affiliations

  1. Institut des Biomoléules Max Mousseron, UMR 5247, CNRS Université Montpellier 1, Université Montpellier 2, Montpellier, France

    François Morvan, Albert Meyer & Jean-Jacques Vasseur

  2. Institut des Nanotechnologies de Lyon – INL, UMR CNRS 5270, Université de Lyon, Site Ecole Centrale de Lyon, Ecully, France

    Yann Chevolot, Jing Zhang & Eliane Souteyrand

  3. Laboratoire de Chimie Organique 2, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Glycochimie, UMR5246, CNRS, Université Lyon 1, Villeurbanne, France

    Sébastien Vidal & Jean-Pierre Praly

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  1. François Morvan
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  2. Yann Chevolot
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  3. Jing Zhang
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  4. Albert Meyer
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  5. Sébastien Vidal
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Correspondence to Yann Chevolot .

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  1. CNRS UMR 5270, Inst. Nanotechnologies de Lyon, Université Lyon 1, av. Guy de Collongue 36, Ecully, 69134, France

    Yann Chevolot

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Morvan, F. et al. (2012). Glycoarray by DNA-Directed Immobilization. In: Chevolot, Y. (eds) Carbohydrate Microarrays. Methods in Molecular Biology, vol 808. Humana Press. https://doi.org/10.1007/978-1-61779-373-8_14

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  • DOI: https://doi.org/10.1007/978-1-61779-373-8_14

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  • Print ISBN: 978-1-61779-372-1

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