Abstract
Polyamine polymers are among the commonest polymers used in biomedicine. Among polyamine polymers, polyethylenimine (PEI) may be used as an efficient delivery vehicle for nucleic acids (DNA, RNA, etc.) or employed as a versatile imaging probe in vivo. In this chapter, the preparation of various PEI bioconjugates will be fully explained and discussed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Vicennati, P., Giuliano, A., Ortaggi, G., and Masotti, A. (2008) Polyethylenimine in medicinal chemistry. Curr. Med. Chem. 15, 2826–2839.
Eliyahu, H., Barenholz, Y., and Domb, A. J. (2005) Polymers for DNA delivery. Molecules 10, 34–64.
Park, T. G., Jeong, J. H., and Kim, S. W. (2006) Current status of polymeric gene delivery systems. Adv. Drug Deliv. Rev. 58, 467–486.
Lemkine, G. F., and Demeneix, B. A. (2001) Polyethylenimines for in vivo gene delivery. Curr. Opin. Mol. Ther. 3, 178–182.
Jere, D., Jiang, H. L., Arote, R., Kim, Y. K., Choi, Y. J., Cho, M. H., Akaike, T., and Cho, C. S. (2009) Degradable polyethylenimines as DNA and small interfering RNA carriers. Expert Opin. Drug Deliv. 6, 827–834.
Masotti, A., Moretti, F., Mancini, F., Russo, G., Di Lauro, N., Checchia, P., Marianecci, C., Carafa, M., Santucci, E., and Ortaggi, G. (2007) Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA. Bioorg. Med. Chem. 15, 1504–1515.
Masotti, A., Vicennati, P., Boschi, F., Calderan, L., Sbarbati, A., and Ortaggi, G. (2008) A novel near-infrared indocyanine dye-polyethylenimine conjugate allows DNA delivery imaging in vivo. Bioconjug. Chem. 19, 983–987.
Boussif, O., Lezoualc’h, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., and Behr, J. P. (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine. Proc. Natl. Acad. Sci. USA. 92, 7297–7301.
Gans, P., and O’Sullivan, B. (2000) GLEE, a new computer program for glass electrode calibration. Talanta 51, 33–37.
North Atlantic Treaty Organization. Scientific Affairs Division. (1998) Near-infrared dyes for high technology applications. In: Daehne, S., Resch-Genger, U., and Wolfbeis, O.S. (Eds.) Proceedings of the NATO Advanced Research Workshop on Syntheses, Optical Properties and Applications of Near-Infrared (NIR) Dyes in High Technology Fields, Trest, Czech Republic, September 24–27, 1997. Kluwer, Dordrecht, London.
Mishra, A., Behera, R. K., Behera, P. K., Mishra, B. K., and Behera, G. B. (2000) Cyanines during the 1990s: A review. Chem. Rev. 100, 1973–2012.
Wiethoff, C. M., Gill, M. L., Koe, G. S., Koe, J. G., and Middaugh, C. R. (2003) A fluorescence study of the structure and accessibility of plasmid DNA condensed with cationic gene delivery vehicles. J. Pharm. Sci. 92, 1272–1285.
Bouteiller, C., Clave, G., Bernardin, A., Chipon, B., Massonneau, M., Renard, P. Y., and Romieu, A. (2007) Novel water-soluble near-infrared cyanine dyes: Synthesis, spectral properties, and use in the preparation of internally quenched fluorescent probes. Bioconjug. Chem. 18, 1303–1317.
Toutchkine, A., Nguyen, D. V., and Hahn, K. M. (2007) Merocyanine dyes with improved photostability. Org. Lett. 9, 2775–2777.
Acknowledgments
The Ministero della Salute and MIUR (Ministero dell’Istruzione, dell’Università e della Ricerca) are gratefully acknowledged for providing financial support for this work. FP also thanks Ernst H.K. Stelzer for support and many interesting discussions.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Masotti, A., Pampaloni, F. (2011). Polyethylenimine Bioconjugates for Imaging and DNA Delivery In Vivo. In: Mark, S. (eds) Bioconjugation Protocols. Methods in Molecular Biology, vol 751. Humana Press. https://doi.org/10.1007/978-1-61779-151-2_10
Download citation
DOI: https://doi.org/10.1007/978-1-61779-151-2_10
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-150-5
Online ISBN: 978-1-61779-151-2
eBook Packages: Springer Protocols