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Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection

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Protein Microarray for Disease Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 723))

Abstract

Recombinant antigen arrays represent a new frontier in parallel analysis of multiple immune response profiles requiring only minute blood samples. In this article, we review the benefits and pitfalls of recombinant antigen microarrays developed for multiplexed antibody quantification. In particular, we describe the development of antigen arrays presenting a set of Y chromosome-encoded antigens, called H-Y antigens. These H-Y antigens are immunologically recognized as minor histocompatibility antigens (mHA) following allogeneic blood and organ transplantation. Clinically relevant B-cell responses against H-Y antigens have been demonstrated in male patients receiving female hematopoietic stem cell grafts and are associated with chronic graft versus host development. This chapter discusses our recombinant antigen microarray methods to measure these clinically relevant allo-antibodies.

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Acknowledgments

This work was supported by NIH R21 HL084318-01A1 and P01 CA049605. We would like to thank Mrs. Fang Wu for her help in processing the H-Y slides. We would also like to thank Dr. John Coller, Director of the Stanford Protein Array Core Facility, for his advice and printing of the H-Y recombinant antigen arrays.

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Authors and Affiliations

  1. Department of Medicine, Blood and Marrow Transplantation Division, Stanford University, Stanford, CA, USA

    Persis P. Wadia, Bita Sahaf & David B. Miklos

Authors
  1. Persis P. Wadia
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  2. Bita Sahaf
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  3. David B. Miklos
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Corresponding author

Correspondence to David B. Miklos .

Editor information

Editors and Affiliations

  1. Cancer Vaccine Center, Department of Medical Oncology, Dana-Farber Cancer Institute, Avenue Louis Pasteur 77, Boston, 02115, Massachusetts, USA

    Catherine J. Wu

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Wadia, P.P., Sahaf, B., Miklos, D.B. (2011). Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection. In: Wu, C. (eds) Protein Microarray for Disease Analysis. Methods in Molecular Biology, vol 723. Humana Press. https://doi.org/10.1007/978-1-61779-043-0_7

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  • DOI: https://doi.org/10.1007/978-1-61779-043-0_7

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-042-3

  • Online ISBN: 978-1-61779-043-0

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