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Posttranscriptional Regulation of Ornithine Decarboxylase

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 720))

Abstract

Activity of the polyamine biosynthetic enzyme ornithine decarboxylase (ODC) and intracellular levels of ODC protein are controlled very tightly. Numerous studies have described ODC regulation at the levels of transcription, translation, and protein degradation in normal cells and dysregulation of these processes in response to oncogenic stimuli. Although posttranscriptional regulation of ODC has been well documented, the RNA binding proteins (RBPs) that interact with ODC mRNA and control synthesis of the ODC protein have not been defined. Using Ras-transformed rat intestinal epithelial cells (Ras12V cells) as a model, we have begun identifying the RBPs that associate with the ODC transcript. Binding of RBPs could potentially regulate ODC synthesis by either changing mRNA stability or rate of mRNA translation. Techniques for measuring RBP binding and translation initiation are described here. Targeting control of ODC translation or mRNA decay could be a valuable method of limiting polyamine accumulation and subsequent tumor development in a variety of cancers.

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Acknowledgements

The authors would like to thank Suzanne Sass-Kuhn for excellent technical assistance. This work was supported by R01 CA82768 and R03 CA142051 (to LMS).

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Authors and Affiliations

  1. Department of Cellular and Molecular Physiology, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, PA, USA

    Shannon L. Nowotarski, Sofia Origanti & Lisa M. Shantz

Authors
  1. Shannon L. Nowotarski
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  2. Sofia Origanti
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  3. Lisa M. Shantz
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Corresponding author

Correspondence to Lisa M. Shantz .

Editor information

Editors and Affiliations

  1. College of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, University Drive 500, Hershey, 17033-0850, Pennsylvania, USA

    Anthony E. Pegg

  2. School of Medicine, Sidney Kimmel Comprehensive, Johns Hopkins University, Orleans St. 1650, Baltimore, 21231, Maryland, USA

    Robert A. Casero, Jr.

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Nowotarski, S.L., Origanti, S., Shantz, L.M. (2011). Posttranscriptional Regulation of Ornithine Decarboxylase. In: Pegg, A., Casero, Jr., R. (eds) Polyamines. Methods in Molecular Biology, vol 720. Humana Press. https://doi.org/10.1007/978-1-61779-034-8_17

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  • DOI: https://doi.org/10.1007/978-1-61779-034-8_17

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-033-1

  • Online ISBN: 978-1-61779-034-8

  • eBook Packages: Springer Protocols

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