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Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture

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Mesenchymal Stem Cell Assays and Applications

Part of the book series: Methods in Molecular Biology ((MIMB,volume 698))

Abstract

The amniotic fluid and placenta are sources of diverse progenitor cell populations, including ­mesenchymal, hematopoietic, trophoblastic, and possibly more primitive stem cells. Given that much of the amniotic cavity and placenta share a common origin, namely the inner cell mass of the morula, perhaps it is not surprising that most types of progenitor cells that can be isolated from these two sources also share many characteristics. This chapter focuses solely on the most abundant and easy to isolate progenitor cell population found therein, the mesenchymal stem cells (MSCs). Unlike some of the other stem cell types, MSCs are present throughout gestation. Methods of isolation, expansion, freezing, and thawing of these cells will be presented with preference given to the simplest methods available for any given procedure.

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Author information

Authors and Affiliations

  1. Department of Surgery, Children’s Hospital Boston and Harvard Medical School, Boston, MA, USA

    Justin D. Klein

Authors
  1. Justin D. Klein
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  2. Dario O. Fauza
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Corresponding author

Correspondence to Justin D. Klein .

Editor information

Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Frederick, 21704, USA

    Mohan Vemuri

  2. Cellular Dynamics International, Inc., Science Drive 525, Madison, 53711, Wisconsin, USA

    Lucas G. Chase

  3. Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mahendra S. Rao

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Klein, J.D., Fauza, D.O. (2011). Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture. In: Vemuri, M., Chase, L., Rao, M. (eds) Mesenchymal Stem Cell Assays and Applications. Methods in Molecular Biology, vol 698. Humana Press. https://doi.org/10.1007/978-1-60761-999-4_7

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  • DOI: https://doi.org/10.1007/978-1-60761-999-4_7

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-998-7

  • Online ISBN: 978-1-60761-999-4

  • eBook Packages: Springer Protocols

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