Abstract
Increasingly, in vitro experiments are being used to evaluate the cell compatibility of novel biomaterials. Single cell cultures have been used to determine how well cells attach, grow, and exhibit characteristic functions on these materials and the outcome of such tests is generally accepted as an indicator of biocompatibility. However, organs and tissues are not made up of one cell type and the interaction of cells is known to be an essential factor for physiological cell function. To more accurately examine biomaterials for bone regeneration, we have developed methods to coculture osteoblasts, which are the primary cell type making up bone, and endothelial cells, which form the vasculature supplying cells in the bone with oxygen and nutrients to survive on 2- and 3-D biomaterials.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kirkpatrick, C. J., Fuchs, S., Iris Hermanns, M., Peters, K., and Unger, R. E. (2007) Cell culture models of higher complexity in tissue engineering and regenerative medicine. Biomaterials 28, 5193–5198.
Campillo-Fernandez, A. J., Unger, R. E., Peters, K., Halstenberg, S., Santos, M., Sanchez, M. S., Duenas, J. M., Pradas, M. M., Ribelles, J. L., and Kirkpatrick, C. J. (2008) Analysis of the biological response of endothelial and fibroblast cells cultured on synthetic scaffolds with various hydrophilic/hydrophobic ratios: Influence of fibronectin adsorption and conformation. Tissue Eng. Part A 5, 1331–1341.
Dubruel, P., Unger, R., Vlierberghe, S. V., Cnudde, V., Jacobs, P. J., Schacht, E., and Kirkpatrick, C. J. (2007) Porous gelatin hydrogels: 2. In vitro cell interaction study. Biomacromolecules 8, 338–344.
Santos, M. I., Fuchs, S., Gomes, M. E., Unger, R. E., Reis, R. L., and Kirkpatrick, C. J. (2007) Response of micro- and macrovascular endothelial cells to starch-based fiber meshes for bone tissue engineering. Biomaterials 28, 240–248.
Santos, M. I., Tuzlakoglu, K., Fuchs, S., Gomes, M. E., Peters, K., Unger, R. E., Piskin, E., Reis, R. L., and Kirkpatrick, C. J. (2008) Endothelial cell colonization and angiogenic potential of combined nano- and micro-fibrous scaffolds for bone tissue engineering. Biomaterials 29, 4306–4313.
Thimm, B. W., Unger, R. E., Neumann, H. G., and Kirkpatrick, C. J. (2008) Biocompatibility studies of endothelial cells on a novel calcium phosphate/SiO2-xerogel composite for bone tissue engineering. Biomed. Mater. 3, 15007.
Unger, R. E., Huang, Q., Peters, K., Protzer, D., Paul, D., and Kirkpatrick, C. J. (2005) Growth of human cells on polyethersulfone (PES) hollow fiber membranes. Biomaterials 26, 1877–1884.
Unger, R. E., Krump-Konvalinkova, V., Peters, K., and Kirkpatrick, C. J. (2002) In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R. Microvasc. Res. 64, 384–397.
Unger, R. E., Peters, K., Huang, Q., Funk, A., Paul, D., and Kirkpatrick, C. J. (2005) Vascularization and gene regulation of human endothelial cells growing on porous polyethersulfone (PES) hollow fiber membranes. Biomaterials 26, 3461–3469.
Unger, R. E., Wolf, M., Peters, K., Motta, A., Migliaresi, C., and James Kirkpatrick, C. (2004) Growth of human cells on a non-woven silk fibroin net: a potential for use in tissue engineering. Biomaterials 25, 1069–1075.
Bondar, B., Fuchs, S., Motta, A., Migliaresi, C., and Kirkpatrick, C. J. (2008) Functionality of endothelial cells on silk fibroin nets: comparative study of micro- and nanometric fibre size. Biomaterials 29, 561–572.
Fuchs, S., Ghanaati, S., Orth, C., Barbeck, M., Kolbe, M., Hofmann, A., Eblenkamp, M., Gomes, M., Reis, R. L., and Kirkpatrick, C. J. (2009) Contribution of outgrowth endothelial cells from human peripheral blood on in vivo vascularization of bone tissue engineered constructs based on starch polycaprolactone scaffolds. Biomaterials 30, 526–534.
Fuchs, S., Hofmann, A., and Kirkpatrick, C. J. (2007) Microvessel-like structures from outgrowth endothelial cells from human peripheral blood in 2-dimensional and 3-dimensional co-cultures with osteoblastic lineage cells. Tissue Eng. 13, 2577–2588.
Fuchs, S., Jiang, X., Schmidt, H., Dohle, E., Ghanaati, S., Orth, C., Hofmann, A., Motta, A., Migliaresi, C., and Kirkpatrick, C. J. (2009) Dynamic processes involved in the pre-vascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells. Biomaterials 30, 1329–1338.
Unger, R. E., Sartoris, A., Peters, K., Motta, A., Migliaresi, C., Kunkel, M., Bulnheim, U., Rychly, J., and Kirkpatrick, C. J. (2007) Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials. Biomaterials 28, 3965–3976.
Acknowledgments
This work was supported by the German Federal Ministry of Education and Research (Ref. Nr. 0313405C) and the NoE EXPERTISSUES (Contract No. 500283-2) from the EU. We also wish to thank DOT GmbH and Curasan AG for generously supplying us with the biomaterials used in these studies.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Unger, R.E., Halstenberg, S., Sartoris, A., Kirkpatrick, C.J. (2011). Human Endothelial and Osteoblast Co-cultures on 3D Biomaterials. In: Haycock, J. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 695. Humana Press. https://doi.org/10.1007/978-1-60761-984-0_15
Download citation
DOI: https://doi.org/10.1007/978-1-60761-984-0_15
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-983-3
Online ISBN: 978-1-60761-984-0
eBook Packages: Springer Protocols