Abstract
Multiplex Ligation-dependent Probe Amplification (MLPA) is a PCR-based technique that was developed for identifying deletions and duplications in genomic DNA. The simplicity and sensitivity of this approach has led to it being implemented in many laboratories around the world. Since the original publication, there have been several variants of MLPA described, allowing the quantitative analysis of mRNA transcript levels, CpG methylation, complex genomic regions, and DNaseI hypersensitive sites. This chapter outlines the basic MLPA protocol, describes the different modifications and applications that have been published, and discusses the critical points during each of the steps.
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Acknowledgments
We thank Dr Jan Schouten and Professor Johan den Dunnen for their helpful discussions. SW is funded by NHMRC Australia (grant numbers 491293 and 546478).
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© 2011 Humana Press
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Ohnesorg, T., Turbitt, E., White, S.J. (2011). The Many Faces of MLPA. In: Park, D. (eds) PCR Protocols. Methods in Molecular Biology, vol 687. Humana Press. https://doi.org/10.1007/978-1-60761-944-4_13
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DOI: https://doi.org/10.1007/978-1-60761-944-4_13
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