Abstract
This chapter contains the description of several methods used for the isolation of functional photosystem II (PS II) core particles from wild type, photosystem I-less, and CP47 histidine-tagged cells of the cyanobacterium Synechocystis sp. PCC 6803. The presented protocols cover cultivation of photosystem I-containing and photosystem I-less cells, isolation of thylakoid membranes, purification of PS II core particles using a weak cation exchange or metal affinity column chromatography, and characterization of the final preparation. These isolation procedures yield highly active oxygen-evolving PS II particles and can be easily adapted for obtaining preparations from Synechocystis mutants with genetically modified photosystem II.
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Acknowledgments
The author would like to thank Dr. Wim Vermaas for his support and Dr. Ilya R. Vassiliev for the absorption spectrum of isolated PS I complex.
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Vavilin, D.V. (2011). Methods for the Isolation of Functional Photosystem II Core Particles from the Cyanobacterium Synechocystis sp. PCC 6803. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods in Molecular Biology, vol 684. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-925-3_4
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DOI: https://doi.org/10.1007/978-1-60761-925-3_4
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