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Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides

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Peptidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 615))

Abstract

A large number of diverse antimicrobial peptides have been found in amphibian skins, and many more remain to be identified. It is sufficiently easy to obtain amounts of gland secretions sufficient for both identification and functional testing of the bioactive peptides. We describe here a systematic peptidomics approach which we combined with genomics and functional testing. This has proven to be an effective way to identify amphibian antimicrobial peptides, including novel peptide families. Protocols are exemplified for Bombina maxima and Odorrana grahami and can be easily adapted for use with other amphibian species.

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Authors and Affiliations

  1. Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China

    Ren Lai

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  1. Ren Lai
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Editors and Affiliations

  1. School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey, TW20 0EX, United Kingdom

    Mikhail Soloviev

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Lai, R. (2010). Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides. In: Soloviev, M. (eds) Peptidomics. Methods in Molecular Biology, vol 615. Humana Press. https://doi.org/10.1007/978-1-60761-535-4_14

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  • DOI: https://doi.org/10.1007/978-1-60761-535-4_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-534-7

  • Online ISBN: 978-1-60761-535-4

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