Abstract
Reporter mice associated to molecular imaging represent a major asset for the study of the spatio-temporal effects of drugs in living animals. The field is still relatively young and so far the number of animals genetically modified to express a given reporter gene ubiquitously and under the control of specific drugs is still limited. For a reporter animal the indispensable elements for the application to drug research and development are (i) the short life of the reporter enabling to have a clear view of the onset as well as the termination of drug effects, (ii) the generalized, drug-dependent activation of the reporter, and (iii) imaging modality suitable for high-throughput analysis. Because of its relative cheapness and ease to perform, in addition to all the above considerations, bioluminescence-based imaging is now regarded as the best imaging technology to be applied to the field of drug research. We show here the application of reporter mouse systems for drug screening in living animals in order to compare drug potency on target and specificity of action.
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Acknowledgment
The authors are grateful to the European Community for the significant and continued contribution to the work here shown with grant support: EDERA (EC-QLK4-CT-2002-02221); NoE DIMI (LSHB CT 2005 512146); NoE EMIL (LSHC CT 2004 503569); Strep EWA (LSHM CT 2005 518245); IP CRESCENDO (LSHM CT 2005 018652).
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Rando, G., Biserni, A., Ciana, P., Maggi, A. (2010). Profiling of Drug Action Using Reporter Mice and Molecular Imaging. In: Proetzel, G., Wiles, M. (eds) Mouse Models for Drug Discovery. Methods in Molecular Biology, vol 602. Humana Press. https://doi.org/10.1007/978-1-60761-058-8_5
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DOI: https://doi.org/10.1007/978-1-60761-058-8_5
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