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References
Sheffield VC, Cox DR, Lerman LS, Meyers RM (1989) Attachment of a 40-base-pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc Natl Acad Sci USA 86:232–236
Wu Y, Hayes VM, Osinga J, et al (1998) Improvement of fragment and primer selection for mutation detection by denaturing gradient gel electrophoresis. Nucleic Acids Res 26:5432–5440
Krawczak M, Reiss J, Cooper DN (1992) The mutational spectrum of single base-pair substitutions in mRNA splice junctions of human genes: causes and consequences Hum Genet 90:41–54
van der Luijt RB, Khan PM, Vasen HF, et al (1997) Molecular analysis of the APC gene in 105 Dutch kindreds with familial adenomatous polyposis: 67 germline mutations identified by DGGE, PTT, and southern analysis. Hum Mutat 9:7–16
Wagner A, Hendriks Y, Meijers-Heijboer EJ, et al (2001) Atypical HNPCC owing to MSH6 germline mutations: analysis of a large Dutch pedigree. J Med Genet 38:318–322
Wijnen J, Vasen H, Khan PM, et al (1995) Seven new mutations in hMSH2, an HNPCC gene, identified by denaturing gradient-gel electrophoresis. Am J Hum Genet 56:1060–1066
den Dunnen JT, Grootscholten PM, Bakker E, et al (1989) Topography of the Duchenne muscular dystrophy (DMD) gene: FIGE and cDNA analysis of 194 cases reveals 115 deletions and 13 duplications. Am J Hum Genet 45:835–847
Hayes VM (2003) Genetic diversity of the alpha- 1-antitrypsin gene in Africans identified using a novel genotyping assay. Hum Mutat 22:59–66
Kerem B, Rommens JM, Buchanan JA, et al (1989) Identification of the cystic fibrosis gene: genetic analysis. Science 245:1073–1080
Soussi T Beroud C (2001) Assessing TP53 status in human tumours to evaluate clinical outcome. Nature Rev Cancer 1:233–240
Nedergaard T, Guldberg P, Ralfkiaer E, Zeuthen J (1997) A one-step DGGE scanning method for detection of mutations in the K-, N-, and H-ras oncogenes: mutations at codons 12, 13 and 61 are rare in B-cell non-Hodgkin's lymphoma. Int J Cancer 71:364–369
Hartmann A, Blaszyk H, McGovern RM, et al (1995) p53 gene mutations inside and outside of exons 5-8: the patterns differ in breast and other cancers. Oncogene 10:681–688
Tannock GW (2002) Analysis of the intestinal microflora using molecular methods Eur J Clin Nutr 56 (Suppl. 4):S44–S49
Favier CF, Vaughan EE, De Vos WM, and Akkermans AD (2002) Molecular monitoring of succession of bacterial communities in human neonates. Appl Environ Microbiol 68:219–226
Donskey CJ, Hujer AM, Das SM, et al (2003) Use of denaturing gradient gel elec-trophoresis for analysis of the stool microbiota of hospitalized patients. J Microbiol Methods 54:249–256
Orita M, Suzuki Y, Sekiya T, Hayashi K (1989) Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5:874–879
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We thank Stefan White for critically reading the manuscript.
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Roelfsema, J.H., Peters, D.J.M. (2008). Denaturing Gradient Gel Electrophoresis (DGGE). In: Walker, J.M., Rapley, R. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-375-6_8
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DOI: https://doi.org/10.1007/978-1-60327-375-6_8
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