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References
Patel AS, Hawkins AL, and Griffin CA (2000) Cytogenetics and cancer. Curr Opin Oncol 12:62–67
Houldsworth J, Chaganti RSK (1994) Comparative genomic hybridization: an overview. Am J Pathol 145:1253–1260
Wells D, Levy B (2003) Cytogenetics in reproductive medicine: the contribution of comparative genomic hybridization (CGH). BioEssays 25:289–300
Boultwood J, Fidler C (eds) (2002) Molecular analysis of cancer, Humana, Totowa, NJ
Kallioniemi A, Kallioniemi OP, Sudar D, et al (1992) Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 258: 818–821
Baksara BR, Pei J, Testa JR (2002) Comparative genomic hybridization analysis. In: Boultwood J, Fidler C (eds) molecular analysis of cancer. Humana, Totowa, NJ, pp. 45–65
Wang PH, Shyong WY, Lin CH, et al (2002) Analysis of genetic aberrations in uterine adenomyosis using comparative genomic hybridization. Anal Quant Cytol Histol. 24:1–6
du Manoir S, Schrock E, Bentz M, et al (1995) Quantitative analysis of comparative genomic hybridization. Cytometry 19:27–41
Piper J, Rutovitz D, Sudar D, et al (1995) Computer image analysis of comparative genomic hybridization. Cytometry 19:10–26
Lundsteen C, Maahr J, Christensen B, et al (1995) Image analysis in comparative genomic hybridization. Cytometry 19:42–50
du Manoir S, Kallioniemi OP, Lichter P, et al. (1995) Hardware and software requirements for quantitative analysis of comparative genomic hybridization. Cytometry 19:4–9
Wells D, Sherlock JK, Handyside AH, and Delhanty JD (1999) Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridization. Nucleic Acids Res. 27: 1214–1218
Kallioniemi OP, Kallioniemi A, Piper J, et al (1994) Optimizing comparative genomic hybridization for analysis of DNA sequences copy number changes in solid tumors. Genes Chromosomes Cancer 10:231–243
Isola J, Devries S, Chu L, Ghazvini S, and Waldman F (1994) Analysis of changes in DNA sequence copy number by comparative genomic hybridization in archival paraffin-embedded tumor samples. Am J Pathol 145:1301–1308
Choo KHA (1994) Methods in molecular biology: in situ hybridization protocols. Humana, Totowa, NJ
Kallioniemi A, Kallioniemi OP, Piper J, et al (1994) Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization. Proc Natl Acad Sci USA 91:2156–160
Kirchhoff M, Gerdes T, Rose H, Maahr J, Ottesen AM, Lundsteen C (1998) Detection of chromosomal gains and losses in comparative genomic hybridization analysis based on standard reference intervals. Cytometry 31:163–173
Tachdjian G, Aboura A, Lapierre JM, Vigie F (2000) Cytogenetic analysis from DNA by comparative genomic hybridization. Ann Genet 43:147–154
Zitzelsberger H, Lehmann L, Wemer M, and Bauchinger M (1997) Comparative genomic hybridisation for the analysis of chromosomal imbalances in solid tumours and haematological malignancies. Histochem Cell Biol 108:403–417
James L, Varley J (1996) Preparation, labeling and detection of DNA from archival tissue sections suitable for comparative genomic hybridization. Chromosome Res 4:163–164
Kuukasjarvi T, Tanner M, Pennanen S, Karhu R, Visakorpi T, Isola J (1997) Optimizing DOP-PCR for universal amplification of small DNA samples in comparative genomic hybridization. Genes Chromosomes Cancer 18:94–101
Knuutila S, Bjorkqvist A, Autio K, et al (1998) DNA copy number amplifications in human neoplasms. Am J Pathol 152:1107–1123
Knuutila S, Aalto Y, Autio K, et al (1999) DNA copy number losses in human neoplasms. Am J Pathol 155:683–694
Knuutila S, Autio K, Aalto Y (2000) Online access to CGH data of DNA sequence copy number changes. Am J Pathol 157:689–690
Struski S, Doco-Fenzy M, Comillet-Lefebvre P (2002) Compilation of published comparative genomic hybridization studies. Cancer Genet Cytogenet 135: 63–90
Wang N (2002) Cytogenetics and molecular genetics of ovarian cancer. Am J Med Genet 115:157–163
Kudoh K, Takano M, Koshikawa T, et al (1999) Gains of 1q21–q22 and 13q12–q14 are potential indicators for resistance to cisplatin-based chemotherapy in ovarian cancer patients. Clin Cancer Res 5:2526–2531
Mills GB, Schmandt R, Gershenson D, Bast RC (1999) Should therapy of ovarian cancer patients be individualized based on underlying genetic defects? Clin Cancer Res 5:2286–2288
Werner CA, Dohner H, Joos S, et al (1997) High-level DNA amplifications are common genetic aberrations in B-cell neoplasms. Am J Pathol 151:335–342
Bryndorf T, Kirchhoff M, Rose H, et al (1995) Comparative genomic hybridization in clinical cytogenetics. Am J Hum Genet 57:1211–1220
Levy B, Dunn TM, Kaffe S, Kardon N, Hirschhom K (1998) Clinical applications of comparative genomic hybridization. Genet Med 1:4–12
Levy B, Dunn TM, Kern JH, Hirschhorn K, Kardon NB (2002) Delineation of the dup5q phenotype by molecular cytogenetic analysis in a patient with dup5q/del 5p (cri du chat). Am J Med Genet 108:192–197
Xu J, Chen Z (2002) Advances in molecular cytogenetics for the evaluation of mental retardation. Am J Med Genet 117:15–24
Shayesteh L, Lu Y, Kuo WL, et al (1999) PIK3CA is implicated as an oncogene in ovarian cancer. Nat Genet 21:99–102
Pinkel D, Segraves R, Sudar D, et al (1998) High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Nature Genet 20:207–211
Solinas-Toldo S, Lampel S, Stilgenbauer S, et al (1997) Matrix-based comparative genomic hybridization: biochips to screen for genomic imbalances. Genes Chromosomes Cancer 20:399–407
Pollack JR, Perou CM, Alizadeh AA, et al (1999) Genome-wide analysis of DNA copy-number changes using cDNA microarrays. Nature Genet 23:41–46
Armour JAL, Barton DE, Cockbum DJ, Taypor GR (2002) The detection of large deletions or duplications in genomic DNA. Hum Mut 20:325–337
Wessendorf S, Fritz B, Wrobel G, et al (2002) Automated screening for genomic imbalances using matrix-based comparative genomic hybridization. Lab Invest 82: 47–60
Snijders AM, Nowak N, Segraves R, et al (2001) Assembly of microarrays for genome-wide measurement of DNA copy number. Nature Genet 29:263–264
Kirchhoff M, Gerdes T, Rose H, Maahr J, Ottesen AM, Lundsteen C, (1998) Detection of chromosomal gains and losses in comparative genomic hybridization analysis based on standard reference intervals. Cytometry 31:163–173
Acknowledgments
We greatly appreciate the technical support of supervisor Dr. Jeremy A. Squire at the Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada (website: http://www.uto-ronto.ca/cancyto/), Miss Wen-Yuann Shyong at the Taipei Veterans General Hospital, and Dr. Wen-Ling Lee at the Department of Medicine, Cheng-Hsin Rehabilitation Center, Taipei, Taiwan. The work, which took place in the authors' laboratories, has been supported by the National Science Council (NSC 92-2314-B-075-115) and Taipei Veterans General Hospital.
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Wang, PH., Chen, YJ., Lin, CH. (2008). Comparative Genomic Hybridization in Clinical and Medical Research. In: Walker, J.M., Rapley, R. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-375-6_15
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DOI: https://doi.org/10.1007/978-1-60327-375-6_15
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