Abstract
Mouse models have been developed to study the pathogenic process of Clostridium difficile infections, first the intestinal colonization and second the toxin production. These models have also been used to test the role of environmental conditions that modulate infection. Different mouse models have been used successfully to study C. difficile infections such as conventional mice, gnotobiotic mouse models including the monoxenic C. difficile mouse model, and the human microbiota-associated mouse model. The advantages and disadvantages of these models are discussed.
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Acknowledgments
The author would like to thank Séverine Péchiné, Alban Le Monnier, and Sandra Hoys for their advice. The author also thanks her colleagues from the U.E.P.S.D., INRA, Jouy-en-Josas, for their help in gnotoxenic experiments and particularly Sylvie Rabot who is responsible for the gnotobiotic animals.
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Collignon, A. (2010). Methods for Working with the Mouse Model. In: Mullany, P., Roberts, A.P. (eds) Clostridium difficile. Methods in Molecular Biology™, vol 646. Humana Press. https://doi.org/10.1007/978-1-60327-365-7_15
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DOI: https://doi.org/10.1007/978-1-60327-365-7_15
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