Abstract
Ca2+ oscillations inside intracellular organelles are important for regulation of functions such as gene expression at the nucleus, respiration at mitochondria or protein processing at the endoplasmic reticulum. Targeted aequorins are excellent calcium probes for subcellular analysis, but single-cell imaging has proven difficult because of low light yield. Here we describe a procedure that combines virus-based expression of targeted aequorins with photon-counting imaging. This methodology allows real-time resolution of changes of cytosolic, mitochondrial or nuclear Ca2+ signals at the single-cell level.
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Acknowledgements
Financial support from the Spanish Ministerio de Educación y Ciencia (grant BFU-2006-60157) is gratefully acknowledged.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Villalobos, C., Alonso, M.T., García-Sancho, J. (2009). Bioluminescence Imaging of Calcium Oscillations Inside Intracellular Organelles. In: Rich, P., Douillet, C. (eds) Bioluminescence. Methods in Molecular Biology™, vol 574. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-321-3_17
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DOI: https://doi.org/10.1007/978-1-60327-321-3_17
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