Summary
Pronuclear injection is a mode of DNA delivery for the production of transgenic animals. We present protocols for the preparation of DNA for microinjection from small or large plasmids and from BACs. We describe the production of one-cell mouse embryos, unfertile males, and pseudo-pregnant females. Finally, we detail the set up and procedures for the microinjection of one-cell embryos and their transfer into foster females.
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References
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Acknowledgements
We wish to thank the members of the Rigby and Krumlauf laboratories at the National Institute for Medical Research, London, and staff of the Transgenics Service at the Mary Lyon Centre for teaching and sharing the tricks and protocol modifications that are compiled in this chapter.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Gardiner, W.J., Teboul, L. (2009). Overexpression Transgenesis in Mouse: Pronuclear Injection. In: Cartwright, E. (eds) Transgenesis Techniques. Methods in Molecular Biology, vol 561. Humana Press. https://doi.org/10.1007/978-1-60327-019-9_8
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DOI: https://doi.org/10.1007/978-1-60327-019-9_8
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Online ISBN: 978-1-60327-019-9
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