Summary
The possibility of generating transgenic animals is of obvious advantage for the analysis of gene function in development and disease. One of the established vertebrate model systems in developmental biology is the amphibian Xenopus laevis. Different techniques have been successfully applied to create Xenopus transgenics; in this chapter, the so-called meganuclease method is described. This technique is not only technically simple, but also comparably efficient and applicable to both Xenopus laevis and Xenopus tropicalis. The commercially available endonuclease I-SceI (meganuclease) mediates the integration of foreign DNA into the frog genome after coinjection into fertilized eggs. Tissue-specific gene expression, as well as germline transmission, has been observed.
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Acknowledgments
This work was supported by funds from the Deutsche Forschungsgemeinschaft to T.P.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Loeber, J., Pan, F.C., Pieler, T. (2009). Generation of Transgenic Frogs. In: Cartwright, E. (eds) Transgenesis Techniques. Methods in Molecular Biology, vol 561. Humana Press. https://doi.org/10.1007/978-1-60327-019-9_4
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DOI: https://doi.org/10.1007/978-1-60327-019-9_4
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