Summary
Circular dichroism (CD) is a well-established technique for the analysis of both protein and DNA structure. The analysis of protein–nucleic acid complexes presents greater challenges, but at wavelengths above 250 nm, the circular dichroism signal from the DNA predominates. Examples are given of the use of CD to examine structural changes to DNA induced by protein binding.
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References
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Cary, P., Kneale, G.G. (2009). Circular Dichroism for the Analysis of Protein-DNA Interactions. In: Leblanc, B., Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology™, vol 543. Humana Press. https://doi.org/10.1007/978-1-60327-015-1_36
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