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Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions

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DNA-Protein Interactions

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 543))

Summary

Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA–protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.

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Acknowledgments

The experimental data included in this chapter were supported by a grant from the National Science and Engineering Research Council of Canada (NSERC) (grant #138624-06) to S.L.G.

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Authors and Affiliations

  1. Oncology and Molecular Endocrinology Research Center, CHUL, Centre Hospitalier Universitaire de Québec and Laval University, 2705 Laurier Blvd, Québec, QC, Canada, G1V 4G2

    Manon Gaudreault, Marie-Eve Gingras, Maryse Lessard, Steeve Leclerc & Sylvain L. Guérin

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  1. Manon Gaudreault
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  2. Marie-Eve Gingras
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  3. Maryse Lessard
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Correspondence to Sylvain L. Guérin .

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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Gaudreault, M., Gingras, ME., Lessard, M., Leclerc, S., Guérin, S.L. (2009). Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions. In: Leblanc, B., Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology™, vol 543. Humana Press. https://doi.org/10.1007/978-1-60327-015-1_2

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    • DOI: https://doi.org/10.1007/978-1-60327-015-1_2

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