Summary
monolithic macroporous hydrogels, “cryogels,” are produced by polymerization in a partially frozen state when the ice crystals perform as a porogen. Cryogels have a unique combination of properties: (i) large (10–100 μm) pores; (ii) minimal non-specific interactions due to the hydrophilic nature of the polymers; (iii) porosities exceeding 80–90%; (iv) good mechanical stability. These properties of cryogels allow for their application for direct capture of extracellularly expressed histidine-tagged protein from the fermentation broth and separation of different cell types.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Galaev, I.Y., Mattiasson, B. (2008). Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels. In: Zachariou, M. (eds) Affinity Chromatography. Methods in Molecular Biology™, vol 421. Humana Press. https://doi.org/10.1007/978-1-59745-582-4_16
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DOI: https://doi.org/10.1007/978-1-59745-582-4_16
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