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Affinity Chromatography

History, Perspectives, Limitations and Prospects

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Affinity Chromatography

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 421))

Summary

Biomolecule separation and purification has until very recently steadfastly remained one of the more empirical aspects of modern biotechnology. Affinity chromatography, one of several types of adsorption chromatography, is particularly suited for the efficient isolation of biomolecules. This technique relies on the adsorbent bed material that has biological affinity for the substance to be isolated. This review is intended to place affinity chromatography in historical perspective and describe the current status, limitations and future prospects for the technique in modern biotechnology.

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Authors and Affiliations

  1. Faculdade de Ciéncias e Tecnologia, Universidade Nova de Lisboa, Portugal

    Ana Cecília A. Roque

  2. Department of Biotechnology, Institute of Biotechnology, University of Cambridge, Cambridge, UK

    Christopher R. Lowe

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  1. Ana Cecília A. Roque
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  2. Christopher R. Lowe
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Editors and Affiliations

  1. BioMarin Pharmaceutical Inc., CA

    Michael Zachariou (Director Project Management) (Director Project Management)

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Roque, A.C.A., Lowe, C.R. (2008). Affinity Chromatography. In: Zachariou, M. (eds) Affinity Chromatography. Methods in Molecular Biology™, vol 421. Humana Press. https://doi.org/10.1007/978-1-59745-582-4_1

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  • DOI: https://doi.org/10.1007/978-1-59745-582-4_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-659-7

  • Online ISBN: 978-1-59745-582-4

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