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Generation of Protein and Cell Microarrays on Functionalized Surfaces

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Biological Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 671))

Abstract

The technique of selective immobilization of biomolecules in defined positions or areas using a simple procedure is essential for various applications such as biosensors, biochips, biomedical microdevices, and tissue engineering. For the generation of biomolecule microarrays, it is necessary to develop a functional surface retaining protein functionality and cell viability, and an efficient patterning tool having flexibility of size and shape.

In this chapter, we have presented the simple tools of protein and cell microarray based on functional-ized surface such as a spotting method with improvement of protein functionality, a functionalized silicon-based surface using photolithography, and an orthogonally polyelectrolyte-coated surface based on soft-lithography.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea

    Yoo Seong Choi

  2. Department of Chemical and Biological Engineering, Chungnam National University, Daejeon, Korea

    Chang-Soo Lee

Authors
  1. Yoo Seong Choi
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  2. Chang-Soo Lee
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Editor information

Editors and Affiliations

  1. , MIT Division of Health Sciences and Tech, Harvard Medical School, Landsdowne St. 65, Cambridge, 02139, Massachusetts, USA

    Ali Khademhosseini

  2. , Department of Mechanical and Aerospace E, Seoul National University, Kwanak-gu, Shinlim-dong 56-1-1, Seoul, 151-742, Korea, Republic of (South Korea)

    Kahp-Yang Suh

  3. Biophage Pharma, Inc., Royalmount Ave. 6100, Montreal, H4P 2R2, Québec, Canada

    Mohammed Zourob

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© 2011 Springer Science+Business Media, LLC

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Choi, Y.S., Lee, CS. (2011). Generation of Protein and Cell Microarrays on Functionalized Surfaces. In: Khademhosseini, A., Suh, KY., Zourob, M. (eds) Biological Microarrays. Methods in Molecular Biology, vol 671. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-551-0_12

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  • DOI: https://doi.org/10.1007/978-1-59745-551-0_12

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-95-4

  • Online ISBN: 978-1-59745-551-0

  • eBook Packages: Springer Protocols

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