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Measuring Molecular Order and Orientation Using Coherent Anti-Stokes Raman Scattering Microscopy

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Methods in Membrane Lipids

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 400))

Abstract

Coherent anti-Stokes Raman scattering microscopy develops rapidly into a powerful technique to image both the chemical composition and physical state in complex samples from biophysics, biology, and the material sciences. This nonlinear vibrational technique increases the signal relative to spontaneous Raman scattering and does not require labeling of the specimen. A theoretical description of the technique is provided and the two major modes of operation: picosecond- and multiplex coherent anti-Stokes Raman scattering are discussed. The potential of the technique is demonstrated with examples of direct measurement of acyl chain order and orientation in lipid monolayers, bilayers, and lipid vesicles.

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Author information

Authors and Affiliations

  1. Biophysics and Microscopy Group, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands

    Hilde A. Rinia & Michiel Müller

  2. FOM Institute for Atomic and Molecular Physics (AMOLF), Amsterdam, The Netherlands

    George W. H. Wurpel

Authors
  1. Hilde A. Rinia
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  2. George W. H. Wurpel
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  3. Michiel Müller
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Editor information

Editors and Affiliations

  1. Department of Pharmacology, The University of Tennessee Health Science Center, Memphis, TN

    Alex M. Dopico

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© 2007 Humana Press Inc.

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Rinia, H.A., Wurpel, G.W.H., Müller, M. (2007). Measuring Molecular Order and Orientation Using Coherent Anti-Stokes Raman Scattering Microscopy. In: Dopico, A.M. (eds) Methods in Membrane Lipids. Methods in Molecular Biology™, vol 400. Humana Press. https://doi.org/10.1007/978-1-59745-519-0_4

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  • DOI: https://doi.org/10.1007/978-1-59745-519-0_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-662-7

  • Online ISBN: 978-1-59745-519-0

  • eBook Packages: Springer Protocols

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