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Retroviral/Lentiviral Transduction and Transformation Assay

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Leukemia

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 538))

Summary

Non-random chromosomal translocations can be found in about half of acute leukaemia patients and mostly lead to either over-expression of proto-oncogenes or creation of novel fusion genes. To assess the oncogenic potential and characterize the underlying mechanisms mediated by these candidate oncoproteins, a retroviral transduction/transformation assay (RTTA) has been successfully employed to study the biological impacts of a number of proto-oncoproteins and novel fusion proteins in primary hematopoietic cells both in vitro and in vivo. To further widen the application of the RTTA, a lentiviral transduction/transformation assay (LTTA) has also been developed to target the most quiescent hematopoietic stem cells (HSCs). This chapter will cover both the RTTA and LTTA for studying candidate oncogenes involved in human leukaemia.

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Acknowledgment

The authors would like to thank Amanda Wilson, Jenny Yeung, and other members of the So’s lab for constructive advice. This work was supported by the Association for International Cancer Research (AICR), Cancer Research UK, the Kay Kendall Leukaemia Fund, Medical Research Council, Wellcome Trust, and Leukaemia Research Fund. Eric So is an AICR fellow and an EMBO young investigator.

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Authors and Affiliations

  1. Haemato-Oncology Section, The Institute of Cancer Research, Brookes Lawley Building, 15 Cotswold Road, Sutton, Greater London, SM2 5NG, UK

    Chi Wai Eric So

Authors
  1. Bernd B. Zeisig
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  2. Chi Wai Eric So
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Corresponding author

Correspondence to Chi Wai Eric So .

Editor information

Editors and Affiliations

  1. King's College London, The University of London, London, UK

    Chi Wai Eric So (Professor and Chair in Leukaemia Biology) (Professor and Chair in Leukaemia Biology)

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Zeisig, B.B., Wai Eric So, C. (2009). Retroviral/Lentiviral Transduction and Transformation Assay. In: Eric So, C.W. (eds) Leukemia. Methods in Molecular Biology™, vol 538. Humana Press. https://doi.org/10.1007/978-1-59745-418-6_10

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  • DOI: https://doi.org/10.1007/978-1-59745-418-6_10

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-989-5

  • Online ISBN: 978-1-59745-418-6

  • eBook Packages: Springer Protocols

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