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Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors

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Animal Cell Biotechnology

Part of the book series: Methods in Biotechnology ((MIBT,volume 24))

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Abstract

Magnetic resonance imaging (MRI) and spectroscopy (MRS) are powerful noninvasive techniques that can be used to monitor the behavior of cells in intensive bioreactor systems. We describe here a number of nuclear magnetic resonance (NMR)-based techniques that have been used successfully to investigate cell growth and distribution, cellular energetics and the porosity to medium flow and linear flow velocity profiles around and across cell layers in perfusion bioreactors. These are important parameters to determine when designing bioreactor systems and operation protocols that optimize cell productivity.

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Author information

Authors and Affiliations

  1. Biochemistry Department, University of Cambridge, Cambridge, UK

    André A. Neves & Kevin M. Brindle

Authors
  1. André A. Neves
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  2. Kevin M. Brindle
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Editor information

Editors and Affiliations

  1. Institute for Bioprocess and Biochemical Engineering, Hamburg University of Technology, Hamburg, Germany

    Ralf Pörtner

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© 2007 Humana Press Inc., Totowa, NJ

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Neves, A.A., Brindle, K.M. (2007). Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Biotechnology, vol 24. Humana Press. https://doi.org/10.1007/978-1-59745-399-8_10

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  • DOI: https://doi.org/10.1007/978-1-59745-399-8_10

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-660-3

  • Online ISBN: 978-1-59745-399-8

  • eBook Packages: Springer Protocols

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