Abstract
Protein microarray is a powerful tool for identifying disease biomarkers and therapeutical targets, and for systematically studying biological pathways with high efficiency. Although the protein microarray platform has been adopted by proteomic research and discovery, what remains problematic is how to maintain the activities and structures of printed proteins on slide surface. With the recent accomplishments in the R & D laboratory, now scientists around the world are able to preserve the biological functions of spotted proteins for high throughput analysis. Full Moon BioSystems (FMB) has developed general guidelines, which helps scientists efficiently prepare forward as well as reverse-phase protein microarray using FMB’s proprietary polymer-coated slides, to obtain reliable and accurate array data with FMB’s unique detection and analysis technology.
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References
DeRisi, J., Penland, L., Brown, P. O., et al. (1996) Use of cDNA microarray to analysis gene expression patterns in human cancer. Nat. Genet. 14, 457–460.
Lockhart, D. J. and Winzelerw, E. A. (2000) Genomics, gene expression and DNA arrays. Nature 405, 827–836.
Grubb, R. L., Calvert, V. S., Wulkunle, J. D., et al. (2003) Signal pathway profiling of prostate cancer using reverse-phase protein arrays. Proteomics 3, 2142–2146.
MacBeath, G. and Schreiber, S. L. (2000) Printing proteins as microarray for high-throughput function determination. Science 289, 1760–1763.
Petricoin, E. F., Ardekani, A. M., Haitt, B. A., et al. (2002) Use of proteomic patterns in serum to identify ovarian cancer. Lancet 359, 572–577.
Albala, J. (2001) Array-based proteomics: the latest chip challenge. Expert Rev. Mol. Diagn. 1, 145–152.
Nishizuka, S., Charboneau, L., Young, L., et al. (2003) Proteomic profiling of the NCI-60 cancer cell lines using new high-density reverse lysate microarrays. Proc. Nalt. Acad. Sci. USA 100, 14,229–14,234.
Paweletz, C. P., Charboneau, L., Bichsel, V. E., et al. (2000) Rapid protein display profiling of cancer progression directly from human tissue using protein biochip. Drug Dev. Res. 49, 34–42.
Paweletz, C. P., Gillespie, J. W., Ornstein, D. K., et al. (2001) Reverse-phase protein microarrays pro-survival pathways at the cancer invasion front. Oncogene 20, 1981–1989.
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© 2007 Humana Press Inc., Totowa, NJ
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Zong, Y., Zhang, S., Chen, HT., Zong, Y., Shi, Y. (2007). Forward-Phase and Reverse-Phase Protein Microarray. In: Rampal, J.B. (eds) Microarrays. Methods in Molecular Biology™, vol 381. Humana Press. https://doi.org/10.1007/978-1-59745-303-5_18
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DOI: https://doi.org/10.1007/978-1-59745-303-5_18
Publisher Name: Humana Press
Print ISBN: 978-1-58829-589-7
Online ISBN: 978-1-59745-303-5
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