Skip to main content

Forward-Phase and Reverse-Phase Protein Microarray

  • Protocol
Microarrays

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 381))

Abstract

Protein microarray is a powerful tool for identifying disease biomarkers and therapeutical targets, and for systematically studying biological pathways with high efficiency. Although the protein microarray platform has been adopted by proteomic research and discovery, what remains problematic is how to maintain the activities and structures of printed proteins on slide surface. With the recent accomplishments in the R & D laboratory, now scientists around the world are able to preserve the biological functions of spotted proteins for high throughput analysis. Full Moon BioSystems (FMB) has developed general guidelines, which helps scientists efficiently prepare forward as well as reverse-phase protein microarray using FMB’s proprietary polymer-coated slides, to obtain reliable and accurate array data with FMB’s unique detection and analysis technology.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. DeRisi, J., Penland, L., Brown, P. O., et al. (1996) Use of cDNA microarray to analysis gene expression patterns in human cancer. Nat. Genet. 14, 457–460.

    Article  CAS  Google Scholar 

  2. Lockhart, D. J. and Winzelerw, E. A. (2000) Genomics, gene expression and DNA arrays. Nature 405, 827–836.

    Article  CAS  Google Scholar 

  3. Grubb, R. L., Calvert, V. S., Wulkunle, J. D., et al. (2003) Signal pathway profiling of prostate cancer using reverse-phase protein arrays. Proteomics 3, 2142–2146.

    Article  CAS  Google Scholar 

  4. MacBeath, G. and Schreiber, S. L. (2000) Printing proteins as microarray for high-throughput function determination. Science 289, 1760–1763.

    CAS  Google Scholar 

  5. Petricoin, E. F., Ardekani, A. M., Haitt, B. A., et al. (2002) Use of proteomic patterns in serum to identify ovarian cancer. Lancet 359, 572–577.

    Article  CAS  Google Scholar 

  6. Albala, J. (2001) Array-based proteomics: the latest chip challenge. Expert Rev. Mol. Diagn. 1, 145–152.

    Article  CAS  Google Scholar 

  7. Nishizuka, S., Charboneau, L., Young, L., et al. (2003) Proteomic profiling of the NCI-60 cancer cell lines using new high-density reverse lysate microarrays. Proc. Nalt. Acad. Sci. USA 100, 14,229–14,234.

    Article  CAS  Google Scholar 

  8. Paweletz, C. P., Charboneau, L., Bichsel, V. E., et al. (2000) Rapid protein display profiling of cancer progression directly from human tissue using protein biochip. Drug Dev. Res. 49, 34–42.

    Article  CAS  Google Scholar 

  9. Paweletz, C. P., Gillespie, J. W., Ornstein, D. K., et al. (2001) Reverse-phase protein microarrays pro-survival pathways at the cancer invasion front. Oncogene 20, 1981–1989.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Zong, Y., Zhang, S., Chen, HT., Zong, Y., Shi, Y. (2007). Forward-Phase and Reverse-Phase Protein Microarray. In: Rampal, J.B. (eds) Microarrays. Methods in Molecular Biology™, vol 381. Humana Press. https://doi.org/10.1007/978-1-59745-303-5_18

Download citation

  • DOI: https://doi.org/10.1007/978-1-59745-303-5_18

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-589-7

  • Online ISBN: 978-1-59745-303-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics