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High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors

A Unique Approach to Metabolomics

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Metabolomics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 358))

Abstract

Metabolomics is the systematic and theoretically comprehensive study of the small molecules that comprise a biological sample, e.g., sera or plasma. The primary analytical tools used in metabolomics are nuclear magnetic resonance and mass spectroscopy. We here address a different tool, high-performance liquid chromatography (HPLC) separations coupled with coulometric electrode array detection. This system has unique advantages, notably sensitivity and high quantitative precision, but also has unique limitations, such as obtaining little structural information on the metabolites of interest and limited scale-up capacity. The system also only detects redox-active compounds, which can be either a benefit or a detriment, depending on the experimental goals and design. Here, we discuss the characteristics of this HPLC/coulometric electrode array system in the context of metabolomics, and then present the method as practiced in our groups.

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Author information

Authors and Affiliations

  1. Dementia Research Service, Burke Medical Research Institute, White Plains, NY

    Bruce S. Kristal & Yevgeniya I. Shurubor

  2. Department of Neuroscience, Weill Medical College of Cornell University, New York, NY

    Bruce S. Kristal

  3. Department of Psychiatry, Duke University Medical Center, Durham, NC

    Rima Kaddurah-Daouk

  4. Department of Systems Biochemistry, Bedford Veterans Administration Medical Center, Bedford, MA

    Wayne R. Matson

Authors
  1. Bruce S. Kristal
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  2. Yevgeniya I. Shurubor
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  3. Rima Kaddurah-Daouk
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  4. Wayne R. Matson
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Editor information

Editors and Affiliations

  1. Department of Metabolic Networks, Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

    Wolfram Weckwerth

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© 2007 Humana Press Inc.

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Kristal, B.S., Shurubor, Y.I., Kaddurah-Daouk, R., Matson, W.R. (2007). High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors. In: Weckwerth, W. (eds) Metabolomics. Methods in Molecular Biology™, vol 358. Humana Press. https://doi.org/10.1007/978-1-59745-244-1_10

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  • DOI: https://doi.org/10.1007/978-1-59745-244-1_10

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-561-3

  • Online ISBN: 978-1-59745-244-1

  • eBook Packages: Springer Protocols

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