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Virus Evolution as a Tool to Study HIV-1 Biology

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HIV Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 485))

Abstract

Mutational analysis of the viral genome is frequently used to study the role of sequence or structural elements in HIV-1 replication. Many laboratories that use this approach have occasionally come across revertant viruses that overcome an introduced defect either by restoration of the original sequence or by the introduction of additional mutations in the viral genome. Similarly, replication of a wild type virus under selective pressure, due to the presence of inhibitors or due to specific culture settings, may result in the appearance of evolved variants that replicate more efficiently under the applied conditions. We have developed in vitro HIV-1 evolution from an anecdotal event to a systematic research tool to study different aspects of the viral replication cycle. In this manuscript, we will briefly review the method of forced virus evolution to study HIV-1 biology and provide several examples that illustrate the power of this method, as it frequently yielded interesting and unexpected information about the mechanism of virus replication.

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Author information

Authors and Affiliations

  1. Laboratory of Experimental Virology, Academic Medical Center, Amsterdam, The Netherlands

    Ben Berkhout

Authors
  1. Ben Berkhout
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  2. Atze T. Das
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Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, NY, USA

    Vinayaka R. Prasad PhD  & Ganjam V. Kalpana PhD  & 

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Berkhout, B., Das, A.T. (2009). Virus Evolution as a Tool to Study HIV-1 Biology. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols. Methods In Molecular Biology™, vol 485. Humana Press. https://doi.org/10.1007/978-1-59745-170-3_30

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  • DOI: https://doi.org/10.1007/978-1-59745-170-3_30

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-859-1

  • Online ISBN: 978-1-59745-170-3

  • eBook Packages: Springer Protocols

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