Abstract
Cloning by somatic cell nuclear transfer is a complex procedure that is dependent on correct interactions between oocyte and donor cell genome. These interactions require minimal insult to either the oocyte or the transplanted nucleus. Available data also indicate that reprogramming the donor cell genome may be slow, so that the cloned embryo expresses genes typical of the donor cell, and thus has different characteristics from normal embryos. Procedures that minimize damage to the donor genome and that address the unique characteristics of the cloned construct should enhance the efficacy of the method.
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References
Chatot, C. L., Ziomek, C. A., Bavister, B. D., Lewis, J. L., and Torres, I. (1989) An improved culture medium supports development of random-bred one-cell mouse embryos in vitro. J. Reprod. Fertil. 86, 679–688.
Kuretake, S., Kimura, Y., Hoshi, K., and Yanagimachi, R. (1996) Fertilization of mouse oocytes injected with isolated sperm heads. Biol. Reprod. 55, 789–795.
Abramczuk, J., Solter, D., and Koprowski, H. (1977) The beneficial effects of EDTA on development of mouse one-cell embryos in chemically defined medium. Dev. Biol. 61, 378–383.
Biggers, J. D., McGinnis, L. K., and Raffin, M. (2000) Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium. Biol. Reprod. 63, 281–293.
Ho, Y., Wigglesworth, K., Eppig, J. J., and Schultz, R. M. (1995) Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Mol. Reprod. Dev. 41, 232–238.
Hogan, B. F., Costantini, F., and Lacy, E. (1986) Manipulating the Mouse Embryo. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 109–110.
Boiani, M., Eckardt, S., Schöler, H. R., and McLaughlin, K. J. (2002) Oct4 distribution and level in mouse clones: consequences for pluripotency. Genes. Dev. 16, 1209–1219.
Chung, Y. G., Ratnam, S., Chaillet, J. R., and Latham, K. E. (2003) Abnormal posttranscriptional gene regulation controlling DNA methyltransferase expression in cloned mouse embryos. Biol. Reprod. 69, 146–153.
Wakayama, T., Perry, A. C. F., Zuccotti, M., Johnson, K. R., and Yanagimachi, R. (1998) Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394, 369–374.
Wakayama, T. and Yanagimachi, R. (1999) Cloning the laboratory mouse. Semin. Cell Dev. Biol. 10, 253–258.
Chung, Y. G., Mann, M. R., Bartolomei, M. S., and Latham, K. E. (2002) Nuclear-cytoplasmic ‘Tug-of War’ during cloning: Effects of somatic cell nuclei on culture medium preferences in the preimplantation cloned mouse embryo. Biol. Reprod. 66, 1178–1184.
Gao, S., Chung, Y. G., Williams, J. W., Riley, J., Moley, K., and Latham, K. E. (2003) Somatic cell-like features of cloned mouse embryos prepared with cultured myoblast nuclei. Biol. Reprod. 69, 48–56.
Heindryckx, B., Rybouchkin, A., Van Der Elst, J., and Dhont, M. (2001) Effect of culture media on in vitro development of cloned mouse embryos. Cloning 3, 41–50.
Latham, K. E. and Schultz, R. M. (2001) Embryonic genome activation, in Frontiers in Bioscience: Molecular and Biochemical Control of Mammalian Preimplantation Development. Frontiers in Bioscience Publications, Tampa, FL, 6, d748–759.
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Chung, Y.G., Gao, S., Latham, K.E. (2006). Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice. In: Verma, P.J., Trounson, A.O. (eds) Nuclear Transfer Protocols. Methods in Molecular Biology™, vol 348. Humana Press. https://doi.org/10.1007/978-1-59745-154-3_7
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DOI: https://doi.org/10.1007/978-1-59745-154-3_7
Publisher Name: Humana Press
Print ISBN: 978-1-58829-280-3
Online ISBN: 978-1-59745-154-3
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