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Nuclear Reprogramming

An Overview

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Nuclear Transfer Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 348))

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Abstract

Nuclear reprogramming is an intriguing phenomenon, in which specialized somatic cells reacquire pluripotency through the global resetting of epigenetic modifications without changes occurring to their nuclear DNA information. The nuclear reprogramming activity retained by unfertilized eggs and embryonic stem cells is readily observable through the techniques of nuclear transplantation and cell fusion, respectively. Furthermore, researches involving somatic stem cells have provided evidence that spontaneous cell fusion functions, at least in part, in maintaining the homeostasis of various tissues through the generation of replacement cells in vivo. Novel approaches by application of the nuclear reprogramming activity may open new avenues producing genetically matched personalized pluripotential stem cells derived from patient somatic cells.

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Author information

Authors and Affiliations

  1. Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto, Japan

    Takashi Tada

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  1. Takashi Tada
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Editor information

Editors and Affiliations

  1. Centre for Reproduction and Development Monash Institute of Medical Research, Monash University, Melbourne, Victoria, Australia

    Paul J. Verma

  2. Monash Immunology and Stem Cell Laboratories, Monash University, Melbourne, Victoria, Australia

    Alan O. Trounson

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© 2006 Humana Press Inc.

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Tada, T. (2006). Nuclear Reprogramming. In: Verma, P.J., Trounson, A.O. (eds) Nuclear Transfer Protocols. Methods in Molecular Biology™, vol 348. Humana Press. https://doi.org/10.1007/978-1-59745-154-3_15

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  • DOI: https://doi.org/10.1007/978-1-59745-154-3_15

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-280-3

  • Online ISBN: 978-1-59745-154-3

  • eBook Packages: Springer Protocols

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