Abstract
Xenopus oocytes have become a favored preparation in which to study the spatiotemporal dynamics of intracellular Ca2+ signaling. Advantages of the oocyte as a model cell system include its large size, lack of intracellular Ca2+ release channels other than the type 1 inositol trisphosphate receptor, and ease of expression of foreign receptors and channels. We describe the use of high-resolution fluorescence imaging techniques to visualize Ca2+ signals in Xenopus oocytes at levels ranging from global Ca2+ waves to single-channel Ca2+ microdomains.
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© 2006 Humana Press Inc., Totowa, NJ
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Dargan, S.L., Demuro, A., Parker, I. (2006). Imaging Ca2+ Signals in Xenopus Oocytes. In: Liu, X.J. (eds) Xenopus Protocols. Methods in Molecular Biology™, vol 322. Humana Press. https://doi.org/10.1007/978-1-59745-000-3_8
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DOI: https://doi.org/10.1007/978-1-59745-000-3_8
Publisher Name: Humana Press
Print ISBN: 978-1-58829-362-6
Online ISBN: 978-1-59745-000-3
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