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Heme•G-Quadruplex DNAzymes: Conditions for Maximizing Their Peroxidase Activity

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G-Quadruplex Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2035))

Abstract

Catalytic DNAs (DNAzymes) with peroxidase-like activity have great potential in bioanalytical chemistry [1], owing to numerous advantages that DNA enzymes offer over conventional protein enzymes, including structural simplicity, low cost, thermal stability, and straightforward handling and preparation. Maximizing the efficiency of the peroxidase activity of such DNAzymes is a subject in need of review. In this chapter, we discuss the optimal experimental conditions for the peroxidase activity of these DNAzymes and describe general procedures for their utilization.

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Acknowledgments

We acknowledge grant funding from the Natural Sciences and Engineering Research Council of Canada (NSERC).

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Authors and Affiliations

  1. Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada

    Nisreen Shumayrikh & Dipankar Sen

  2. Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada

    Dipankar Sen

Authors
  1. Nisreen Shumayrikh
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  2. Dipankar Sen
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Corresponding author

Correspondence to Dipankar Sen .

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Editors and Affiliations

  1. Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Purdue Center for Cancer Research, Purdue Institute for Drug Discovery, West Lafayette, IN, USA

    Danzhou Yang

  2. Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Clement Lin

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Shumayrikh, N., Sen, D. (2019). Heme•G-Quadruplex DNAzymes: Conditions for Maximizing Their Peroxidase Activity. In: Yang, D., Lin, C. (eds) G-Quadruplex Nucleic Acids. Methods in Molecular Biology, vol 2035. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9666-7_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9666-7_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9665-0

  • Online ISBN: 978-1-4939-9666-7

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