Abstract
The success of a proteomics experiment critically depends on the correct execution of the sample preparation. Gel-based sample preparation is a well-proven method widely used in qualitative and quantitative proteomics. Recently, sample preparation in a single vessel has gained popularity because of the ease to perform, the possibility for large-scale analysis, and the potential to improve sensitivity. In this chapter I describe two sample preparation protocols, filter-aided sample preparation (FASP) and single-pot solid-phase sample preparation (SP3).
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Li, K.W. (2019). Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3). In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 146. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9662-9_6
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DOI: https://doi.org/10.1007/978-1-4939-9662-9_6
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Publisher Name: Humana, New York, NY
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